华北农学报Issue(5)：74-79,6.
猪传染性胃肠炎病毒江苏株的分离与鉴定及其S基因序列分析
Isolation and Identification of Porcine Transmissible Gastroenteritis Virus JS2012 Strain and Clone and Sequence Analysis of Its S Complete Genome
摘要
Abstract
By serial passaging on ST cells ,a transmissible gastroenteritis virus (TGEV)strain(TGEV JS2012)was obtained from intestinal contents of died piglets with clinical diarrhea symptom .This virus was proved to be TGEV by RT-PCR,direct immunofluorescence and animal regression test .The S gene of TGEV JS2012 strain primary aassages (P1TS)and the fiftith passages(P50TS),were amplified by PCR and then sequenced .Comparison of the S gene se-quences of P1TS and P50TS with 18 published TGEV strains with different origins showed that they can share 96.0%-99.9%homology of nucleotide sequences and 96 .5%-99 .9%homology of amino acids .Furthermore ,the results of the phylogenetic tree analysis demonstrated that there was a close genetic relationship between P 1 TS strain and HN2002 or Miller M6 strain isolated from China and America respectively .Generally the result indicated the varia-tion of S gene between strains of different origins was not so obvious .The result also showed that a deletion of six bases happened in 1 124-1 129 location of the S gene of P50TS seemed to be similar to Purdue P115 strain which is an attenuated strain ,indicating this deletion perhaps is related to the reduced virulence of TGEV .关键词
TGEV/分离鉴定/S基因/序列分析/缺失Key words
TGEV/Isolation and identification/S Complete genome/Sequence analysis/Deletion分类
农业科技引用本文复制引用
郭容利,倪艳秀,温立斌,李彬,王小敏,俞正玉,何孔旺..猪传染性胃肠炎病毒江苏株的分离与鉴定及其S基因序列分析[J].华北农学报,2013,(5):74-79,6.基金项目
江苏省农业科技自主创新资金项目（CX（12）3076） （）
