华北农学报Issue(6)：11-17,7.
利用染色体单片段代换系定位水稻粒重 QTL
Mapping of QTLs for Grain Weight Using Chromosome Single Segment Substitution Lines in Rice
摘要
Abstract
Grain weight is one of major factors determining rice yield ,which is a typical quantitative trait con-trolled by multiple genes .With Guangluai 4 as recipient and Nipponbare as donor ,a population of 119 chromosome segment substitution lines had been developed .Correlation analysis between grain weight and grain shape by SPSS revealed that 1000-grain weight was significantly and positively correlated with grain length and length -width ratio , while showed no correlation with grain width and thickness .The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett′s test.Nineteen stable QTLs for grain weight were identified over two years.All 19 QTLs were identified on all chromosomes except for chromosome 10 and 12 with a significant level of P≤0.001.Among them,10 QTLs had a positive effect and derived from the Nipponbare allele ,the additive effect of these QTLs ranged from 0.49 g to 2.74 g,the additive effect percentages ranged from 2.00%to 11.05%.Addition-al 9 QTLs had a negative effect and were all derived from Guangluai 4 alleles,the additive effect of these QTLs ranged from 0.60 g to 2.35 g,the additive effect percentages ranged from 2.40%to 9.84%.The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight .关键词
水稻/单片段代换系/数量性状/千粒质量/代换作图Key words
Rice/Chromosome single segment substitution lines/Quantitative trait loci/1000-grain weight/Substitution mapping分类
农业科技引用本文复制引用
王军,周勇,杨杰,朱金燕,范方军,李文奇,梁国华,仲维功..利用染色体单片段代换系定位水稻粒重 QTL[J].华北农学报,2013,(6):11-17,7.基金项目
国家自然科学基金项目（31101131）；国家科技支撑计划重大项目（2011BAD16B03）；江苏省科技支撑计划项目（BE2012309）；江苏省自然科学基金项目（BK2011666）；高等学校博士学科点专项科研基金项目（20103250110004）；江苏省农业自主创新资金项目［CX（12）1003］ （）
