华北农学报Issue(6)：65-70,6.
蔊菜病毒诱导基因沉默体系构建
Virus-induced Gene Silencing in Rorippa indica Hiern
摘要
Abstract
Virus-induced gene silencing system of Rorippa indica Hiern was constructed ,which will be used in functional analysis of R.indica genes.Cloroplasto alterados( CLA) gene fragments of cotton ,soybean and Arabidop-sis were obtained by using RT-PCR.The cloned CLA fragments were constructed into tobacco rattle virus vector (pYY13).The constructed vectors were transformed into Agrobacterium strain GV3101,which was used to inoculate R.indica plants.All the infected plants showed photobleaching symptom with different degrees .RT-PCR of target genes further confirmed the knock-down of CLA.The established VIGS system will contribute the further study of R. indica genes in the future research .关键词
病毒诱导的基因沉默/蔊菜/CLA基因/烟草脆裂病毒Key words
Virus induced gene silencing/Rorippa indica Hiern/CLA gene/Tobacco rattle virus分类
生物科学引用本文复制引用
张怡,徐克东,杨松,李豪,曹鹏,魏森,韦丹丹,李成伟..蔊菜病毒诱导基因沉默体系构建[J].华北农学报,2013,(6):65-70,6.基金项目
国家自然科学基金项目 （）
