华北农学报Issue(6)：77-81,5.
猪圆环病毒2型2010 AHCY 株的分离鉴定及遗传变异分析
Isolation,Identification and Genetic Variation of Porcine Circovirus Type 2 Isolate 2010 AHCY
摘要
Abstract
The aim of this study was to obtain the porcine circovirus type 2(PCV2) isolate 2010AHCY and to identify its biological characteristics ,and to analyze the 50%tissue culture infective dose ( TCID50 ) and the whole genome of this isolate .The samples which were detected PCV 1 negative and PCV2 positive were inoculated and pas-saged on PK15 cells.The isolate was identified by PCR and confirmed by immunofluorescent assay ( IFA).The TCID50 of 2010 AHCY isolate was determined and the whole genome was sequenced and its molecular variations was analyzed by DNAStar software .A PCV2 strain 2010 AHCY was successfully isolated from samples .The results showed that the genome length of 2010AHCY was 1 767 bp and the TCID50 was 104.23.Comparison analysis of the complete genomic sequences of 2010 AHCY and other strains indicated that the homologies among these 11 strains were 93.2%-99.9%and the highest homology of 99.9%to the HM038030 PCV2d strain.The phylogenetic trees revealed that the isolate 2010 AHCY belonged to genotype PCV 2 d and had a close relationship with PCV 2 b geno-type.This study provide foundations for studying the molecular epidemiology ,variation and prevention of PMWS in Eastern China .关键词
猪圆环病毒2型/分离/鉴定/遗传变异Key words
Porcine circovirus type 2(PCV2)/Isolation/Identification/Genetic variation分类
生物科学引用本文复制引用
王小敏,张雪寒,郭容利,李彬,何孔旺,王东田,周忠涛,茅爱华,俞正玉,汪伟,倪艳秀,温立斌..猪圆环病毒2型2010 AHCY 株的分离鉴定及遗传变异分析[J].华北农学报,2013,(6):77-81,5.基金项目
国家基金面上项目（31272574）；江苏省自主创新专项（CX（11）2060） （）
