华北农学报Issue(6)：93-97,5.
拟南芥AtHAK5基因启动子顺式作用元件功能域的鉴定
Identification of Cis-element Location in AtHAK5 Promoter
摘要
Abstract
AtHAK5 encodes a high affinity K +transporter .Six AtHAK5 promoter fragments with different length were cloned into the upstream of β-Glucuronidase reporter gene ( GUS) in pORE R1 binary expression vector ,which forms promoter AtHAK5∶∶GUS fusion gene .The T3 generation transformed seeds stably expressing the fusion gene were gotten through Agrobacterium-mediated Arabidopsis transformation with floral dip method .The cis-elements of the AtHAK5 promoter was located at the transcription region of 471-267 thought GUS activity based histochemical staining of the transformed seedlings grown on low potassium condition .This study identified the functional domain within the AtHAK5 promoter,which sets the foundation for further finding of the corresponding transcript factor .关键词
拟南芥/AtHAK5基因/启动子/顺式作用元件Key words
Arabidopsis/AtHAK5 gene/Promoter/Cis-element分类
农业科技引用本文复制引用
王欣,杨菊,祁智,张彦桃..拟南芥AtHAK5基因启动子顺式作用元件功能域的鉴定[J].华北农学报,2013,(6):93-97,5.基金项目
内蒙古自治区科技厅自然科学基金面上项目 （）
