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37份洋葱遗传多样性的 RAPD 和 SSR 分析

翟亚辉 马蓉丽 成妍 吴海涛 张光星

华北农学报Issue(6):115-120,6.
华北农学报Issue(6):115-120,6.

37份洋葱遗传多样性的 RAPD 和 SSR 分析

Genetic Polymorphism of 37 Onion Germplasm Resources as Revealed by RAPDs and SSRs Markers

翟亚辉 1马蓉丽 2成妍 2吴海涛 2张光星1

作者信息

  • 1. 山西农业大学 园艺学院,山西 太谷 030801
  • 2. 山西省农业科学院 蔬菜研究所,山西 太原 030031
  • 折叠

摘要

Abstract

In this paper,the genetic diversity of 37 onions(Allium cepa L.) germplasm resources were analyzed by SSRs and RAPDs markers and the results of these two methods were compared .The results showed that 61 poly-morphic bands were detected by 15 RAPD primers and 41 polymorphic bands were detected by 8 SSR primers .Both RAPDs and SSRs were highly polymorphic .The percentage of polymorphic RAPDs bands was 84 .7%.The average effective number of alleles was 3.75 for SSRs.The average effective number of alleles ,Nei′s genetic diversity index , Shannon information index for SSRs markers were higher than those in RAPDs .UPGMA cluster analysis indicated that both the two markers could divided 37 germplasm into four groups and the classification were fundamentally i-dentical .The study suggested that both RAPDs and SSRs could be used to analyze the genetic diversity of onion germplasm resources ,but the effect for SSRs marker was better than RAPDs .

关键词

洋葱/RAPD/SSR/遗传多样性

Key words

Onion/RAPD/SSR/Genetic diversity

分类

农业科技

引用本文复制引用

翟亚辉,马蓉丽,成妍,吴海涛,张光星..37份洋葱遗传多样性的 RAPD 和 SSR 分析[J].华北农学报,2013,(6):115-120,6.

基金项目

国家公益性行业(农业)科研专项(20090318-7-3);山西省自然科学基金项目 ()

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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