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马蔺种质资源遗传多样性的 ISSR 分析

毛培春 孟林 田小霞

华北农学报Issue(6):130-135,6.
华北农学报Issue(6):130-135,6.

马蔺种质资源遗传多样性的 ISSR 分析

Genetic Diversity Analysis of Iris lactea var.chinensis Accessions by ISSR Technology

毛培春 1孟林 1田小霞1

作者信息

  • 1. 北京市农林科学院 北京草业与环境研究发展中心,北京 100097
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摘要

Abstract

Genetic diversity of 20 Iris lactea Pall.var.chinensis ( Fisch.) Koidz.,which were collected from 4 provinces or autonomous regions in northern China ,was analyzed using inter-simple sequence repeat (ISSR) meth-od.303 bands were amplified by 14 screened polymorphic primers ,of which 241 bands were polymorphic .Percent-age of polymorphic bands ( PPB ) was about 77 .29%.The range of the genetic similarity coefficient ( GS ) value was 0.340 2~0.824 7 and showed larger scale of distribution ,and showed further that they have the rich genetic diversity.20 accessions of I.lactea were divided into 3 groups at 0.587 6 of GS value using the unweighted pair-group method with arithmetic means ( UPGMA) .The results obtained from UPGMA were consisted with the princi-pal component analysis ( PCA) ,and indicated that the genetic distance of 20 accessions of I.lactea was correlated with the geographical distance .

关键词

马蔺/ISSR/遗传多样性

Key words

Iris lactea Pall.var.chinensis ( Fisch.) Koidz./ISSR/Genetic diversity

分类

农业科技

引用本文复制引用

毛培春,孟林,田小霞..马蔺种质资源遗传多样性的 ISSR 分析[J].华北农学报,2013,(6):130-135,6.

基金项目

北京市自然科学基金项目 ()

华北农学报

OA北大核心CSCDCSTPCD

1000-7091

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