华北农学报Issue(6)：174-180,7.
笃斯越桔花色苷抗禽白血病病毒A亚群活性的试验研究
Study on the Effect of Anthocyanin from Vaccinium uliginosum L.Resistance Avian Leukosis Virus Subgroup A Activity
摘要
Abstract
To investigate whether the anthocyanins from Vaccinium uliginosum L.( AVU) could inhibit Avian Leukosis Virus subgroup A ( ALV-A) on the DF-1 cell by in vitro studies .The anthocyanins structure of sample was i-dentified by HPLC-MS.The DF-1 cells were pretreated with the ALV-A for 1 hour and then exposed to the low dose of the extracts of the anthocyanins for an additional 24 to 96 hours.The cell viability was determined by MTT assay .The DF-1 morphological changes were observed .We found that the extracts of Vaccinium uliginosum L.contained six major anthocyanins ,the one of the main component was cyaniding-3-glucoside .The 40 to 80μg/mL AVU resulted in inhibi-tion of Avian Leukosis Virus subgroup A infection and reduction of apoptosis compared with control .Moreover , we found that 60μg/mL AVU was significantly inhibited the activity of the ALV-A and increased the growth of DF-1 cells.关键词
笃斯越桔/花色苷/DF-1鸡成纤维细胞/禽白血病病毒A亚群/生物活性Key words
Vaccinium uliginosum L./Anthocyanin/DF-1 cell/ALV-A/Biological activity分类
轻工纺织引用本文复制引用
张莉,雷用东,盖丽丽,赵晓燕,童军茂,王丹,马越,张超..笃斯越桔花色苷抗禽白血病病毒A亚群活性的试验研究[J].华北农学报,2013,(6):174-180,7.基金项目
自然科学基金资助项目 （）
