华北农学报Issue(z1)：16-21,6.
吉富品系尼罗罗非鱼抗病性状分子标记筛选及遗传多样性分析
Screen of Disease-resistance Molecular Markers and Analysis of Genetic Diversity in GIFT Strain Nile Tilapia
摘要
Abstract
Technology with microsatellite was used to analyze the genetic difference between disease -resistant and disease-susceptible groups of GIFT strain .The results show that there were 20 loci successfully amplified with stable target bands within 30 tested loci to identify the polymorphism for the tested GIFT strain .The average alleles in disease-resistant was 2.5 slightly higher than that of 2.4 in disease-susceptible group .Then the average of ob-served heterozygosity(Ho)and expected heterozygosity(He)were 0.469 and 0.441,0.440 and 0.476 respectively, and the polymorphism information content (PIC)was 0.359 and 0.379.Genetic similarity index and genetic distance was 0.055 and 0.054 respectively.The locus UNH845,UNH189 and GM180 showed significant departure from Hardy-Weinberg equilibrium after sequential bonferroni correction (P<0.05)in two groups.GM251 and GM462 sites differences of allele fragment (135,198 bp) were positively related to disease-resistance GIFT strain.Above mentioned results indicated that the genetic diversity between disease-resistant and disease-susceptible group of GIFT strain was not significantly different ,and the GM251 and GM462 gene fragment with its differential bands from the PCR ampliftcation can be used as the molecular marker for the disease-resistant GIFT strain molecular assistant selection .关键词
吉富品系尼罗罗非鱼/微卫星/遗传多样性/遗传标记Key words
GIFT strain/Microsatellite/Genetic diversity/Genetic marker分类
生物科学引用本文复制引用
朱佳杰,周宇,沈夏霜,李莉,罗永巨,甘西..吉富品系尼罗罗非鱼抗病性状分子标记筛选及遗传多样性分析[J].华北农学报,2013,(z1):16-21,6.基金项目
广西区水畜牧兽医局科研计划项目（桂畜牧科1204903）；广西壮族自治区公益性科研院所专项基金（ GXIF-2012-15）；国家现代农业产业技术体系建设专项（ CARS-49）；广西八桂学者建设工程专项 （）
