华北农学报Issue(z1)：97-102,6.
白眉蝮蛇降纤酶的同源模建及B细胞抗原表位预测
Homology Modeling and B Cell Antigenic Epitopes Predicition of Defibrase from Agkistrodon hadys ussuriensis Snake Venom
摘要
Abstract
Defibrase from Agkistrodon halys ussuriensis snake venom is essential medicine to be used in the clinical treatment of thromboembilic disease in our country .However,this enzyme derived from snake venom ,is a heterologous protein and is a macromolecular protein ,and therefore ,it's immunogenicity problem is inevitable .More-over,this protein drug was used in large scale in our country ,and therefore,the security issues caused by immunoge-nicity cannot be ignored .In this work,B cell linear epitopes were predicted using DNAStar soft ware and ABCpred , BCPREDS,BcePred,BepiPred 1.0 and Ellipro web servers.B-cell linear epitopes in defibrase were predicted to lo-cate in the regions of 27-35,44-50,66-72,79-98,101-109,117-127,134-142,151-164,179-188,194-206,221-233 and 247-260.Using protein C activator from Agkistrodon contortrix venom(ACC-C) as template,a 3D structure model of defibrase was constructed by Pythonwin program using modeling package MODELLER 9 .10 .Ste-reochemical quality of the selected model was evaluated using PROCHECK ,Errat and PROSA programs .The confor-mational epitopes of defibrase was predicted by 3D structure of homology modeling combined with Discotope 1.2 server and cons PPISP online tools .B-cell conformational epitopes in defibrase were predicted to locate in the re-gions of 45-51 ,81-90 ,95-106 ,134-141 ,153-159 ,177-182 ,225-232 and 251-257 .The expected results of the project are useful for providing new ideas for the safety applications of defibrase drugs ,and providing the tech-nical basis for immunogenicity mechanism of protein drugs .关键词
降纤酶/免疫原性/线性表位/构象型表位/同源模建/长白山白眉蝮蛇Key words
Defibrase/Immunogenicity/Linear epitopes/Conformational eptiopes/Homology modeling/Agkistr-odon halys ussuriensis分类
医药卫生引用本文复制引用
贾秋磊,王林,陈鹏,杨青,姜秀萍..白眉蝮蛇降纤酶的同源模建及B细胞抗原表位预测[J].华北农学报,2013,(z1):97-102,6.基金项目
国家自然科学基金项目（81102378）；中央高校基本科研业务费专项资金 （）
