华北农学报Issue(1)：31-35,5.
山定子MbFAD2基因的克隆及表达分析
Cloning and Expression Analysis of MbFAD2 Gene in Malus baccata
摘要
Abstract
The cDNA sequence of MbFAD2 was cloned from Malus baccata by RT-PCR. It′s open reading frame possesses 1 149 bp,and encodes a peptide of 382 amino acids. It has a calculated molecular mass of 97. 511 2 kDa and a theoretical PI of 5 . 01 . The gene was named as MbFAD2 , and its accession nucleotide sequence number in GenBank was KC702671 . Secondary structure analysis showed that MbFAD2 protein contains 35 . 86% α-helical do-mains,16. 23% extended strand,and 47. 91% random coil. Phylogenetic tree analyses showed that MbFAD2 is clo-sest to the Populus tomentosa and Hevea brasiliensis,the farthest is Caragana korshinskii. The expression of MbFAD2 was determined by real-time quantitative RT-PCR. The result showed than the MbFAD2 was expressed in different tissue organs. The highest mRNA expression was found in flower,secondly is leaf. The MbFAD2 expression could be significantly induced by low temperature(4 ℃) for 24 h,but the stem is difference. These results suggest that Mb-FAD2 may be involved in low temperature.关键词
山定子/MbFAD2/低温处理/表达分析Key words
Malus baccata/MbFAD2/Low temperature treatment/Expression analysis分类
生物科学引用本文复制引用
刘修丽,卢静静,周爱琴,张玉刚,祝军,戴洪义..山定子MbFAD2基因的克隆及表达分析[J].华北农学报,2014,(1):31-35,5.基金项目
国家苹果产业技术体系项目(CARS280107) （CARS280107）
“十二五”国家科技支撑计划项目(2013BAD02B00) （2013BAD02B00）
中央财政农业技术推广项目 （）
山东省良种工程项目 （）
山东省优秀中青年科学家奖励基金项目 （）
青岛市国际科技合作项目 （）
