华北农学报Issue(1)：36-40,5.
马铃薯中VIGS沉默体系的研究
The Effects of Different Potato Genotypes and Different Inoculation Methods on VIGS Silence Efficiency
摘要
Abstract
In this study,factors which affect the silence system of VIGS in potato was unravel,the experiment results suggested that the genotype is the main factor to affect the result of VIGS. Among all the tested potato varie-ties,VIGS showed the highest silence effect in potato variety Wumeng 601,the pds gene expression level was de-cressed by 79. 8%;and the pds gene expression level in Hutou,Shepody and Atlantic was decreased by 64. 1%, 31. 0% and 4. 5% respectively. The effects of inoculation ways such as leaf injection and stem scrub on the silence result of VIGS was also tested,the results suggested that the correlation between inoculation ways and silence effect was not very strong. In Wumeng 601,the pds gene expression level of leaf injection and stem scrub were decreased by 59. 0% and 56. 5% separately;it is 34. 7% and 40. 3% respectively in potato variety Hutou. The analysis of va-riance ( ANOVA) showed there does not have significant difference between the two inoculation ways.关键词
VIGS/沉默/马铃薯/接种方法Key words
VIGS/Silence/Potato varieties/Inoculation method分类
生物科学引用本文复制引用
张晓萝,张之为,娜仁,杨叔清,高婧,赵君..马铃薯中VIGS沉默体系的研究[J].华北农学报,2014,(1):36-40,5.基金项目
国家自然科学基金项目(30760132) （30760132）
