华北农学报Issue(1)：83-88,6.
与蓖麻果刺性状连锁的RAPD标记
The RAPD Marker of Fruit Thorn Traits for Castor
摘要
Abstract
We selected nine fruit thorns and five fruit non-thorns of castor as the research object using the RAPD marker for the thorn traits analysis. The results were as follows:the RAPD-PCR reaction system with opti-mized by single factor was 0. 9 μL of Taq polymerase,2. 5 μL of Taq polymerase 10 × Buffer,2. 5 μL of each dNTP,1. 3 μL of Mg2+,1. 4 μL of 10 pmol/L primer SBS126,1. 4 μL of DNA,ddH2 O 15. 0 μL,Total 25 μL;And the related amplification condition was 1 cycle of 4 min 30 s at 94 ℃;35 cycles of 45 s at 94 ℃,45 s at 38 ℃and 45 s at 72 ℃,followed by a final cycle of 5 min at 72 ℃,then save in 4 ℃. Using the optimized reaction system and condition,several 1 800 bp fragments amplified by SBS126 was found in all the fruit thorn material,and sequen-cing results showed that the upstream homology of fragment was very low,whereas the downstream has the identical sequence with about 112 bp length. Basing on these results,we deduced that the development of fruit thorn may be associated with histidine-containing phosphotransfer protein 2,and this laid a foundation for research on the molecu-lar mechanism of fruit thorn traits for castor.关键词
蓖麻/果刺/RAPD/磷酸转移蛋白Key words
Castor/Fruit thorn/RAPD/Phosphotransfer protein分类
农业科技引用本文复制引用
黄凤兰,赵永,彭木,张智勇,陈晓凤,包春光,邹千稳,吴春桃..与蓖麻果刺性状连锁的RAPD标记[J].华北农学报,2014,(1):83-88,6.基金项目
国家自然科学基金项目(30760123 （）
31160290 （）
31060194) （）
国家民委项目(10NM02) （10NM02）
内蒙古自然科学基金项目(2010BS0511) （2010BS0511）
内蒙古人才基金项目 （）
内蒙古民族大学市校合作项目(SXZD2012018) （SXZD2012018）
内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(BMYJ2011009) （BMYJ2011009）
