华北农学报Issue(1):98-102,5.
大麦苗期根系和茎叶性状的QTL定位分析
QTL Mapping of Root and Shoot Traits at Seedling Stage in Barley
摘要
Abstract
A population of DH was constructed,which derived from a cross between Taixing 9425 with water-logging tolerance and Franklin which is susceptible to waterlogging. The five characters associated with waterlogging tolerance including maximum root length,root fresh weight,root dry weight,shoot fresh weight,shoot dry weight were investigated under water stress. Transgressive segregations for all traits were observed in the population, and their frequencies were showed a continuous distribution. The characters were controlled by multi-genes with major effects, and minor modifiers. Based on the linkage map,the composite interval mapping approach of QTL Cartographer 2. 5 was used to detect the QTL for these traits. A total of 13 QTL associated with root and shoot traits were mapped on all chromosomes except 2 and 3. All QTL were responsible for interpreting 10. 99% -20. 7%,and the range of LOD were 2. 59-3. 83. Among these QTL,2 QTL controlling maximum root length,2 QTL controlling root fresh weight, 3 QTL controlling root dry weight,3 QTL controlling shoot fresh weight and 3 QTL controlling shoot dry weight were detected. Tightly linked QTL regions on chromosome 6 and 7 were identified,which suggested the existence of pleio-tropic or genetic linkage. The above result played a vital role in marker assisted selection and fine mapping of QTL associated with root and waterlogging tolerance.关键词
大麦/苗期/加倍单倍体( DH)群体/数量性状基因座( QTL)/根系和茎叶性状Key words
Barley/Seedling stage/Double haploid line population/Quantitative trait locus/Root and shoot traits分类
农业科技引用本文复制引用
栾海业,许如根,吕超,张新忠,陈和,陈健,沈会权,陶红,乔海龙,臧慧..大麦苗期根系和茎叶性状的QTL定位分析[J].华北农学报,2014,(1):98-102,5.基金项目
国家自然科学基金项目(30671298 ()
30971779 ()
31071407) ()
国家大麦青稞产业技术体系项目(CARS-05) (CARS-05)
江苏省农业科技自主创新资金项目(CX(12)3067) (CX(12)
江苏沿海地区农业科学研究所基金项目(YHS201102) (YHS201102)