华北农学报Issue(1)：208-212,5.
与黄瓜抗黑斑病基因连锁的SCAR标记及抗病资源筛选
SCAR Marker Resistance-related to Cucumber Alternaria Leaf Spot and Resistance Identification of Cucumber Germplasm
摘要
Abstract
In order to establish molecular marker-assisted selection system of cucumber alternaria leaf spot with F1,F2and BC1population between a susceptible parent(L 63) and a resistant parent(L 9),a co-dominant AFLP marker was successfully converted into a simple, applied and co-dominant SCAR marker. Special marker SCEM 126/122 were designed according to the sequence information of AFLP( E-CC/M-CAT) fragments. The marker was closely linked to the cucumber alternaria leaf spot resistance-related gene, and the genetic distance between the marker and the gene was 4. 4 cM. The marker could be useful in marker-assisted selection in cucumber breeding. The acquired SCAR marker had many advantages including fast,accurate,low cost,free from an environmental in-fluence et. 64 resistant germplasm were abtained from total 290. Application of the acquired marker will inhance cu-cumber alternaria leaf spot disease resistant germplasm evaluation.关键词
黄瓜/黑斑病/SCAR/资源Key words
Cucumber/Alternaria leaf spot/SCAR/Germplasm分类
农业科技引用本文复制引用
王惠哲,李淑菊,杨瑞环,管炜,邓强,曹明明..与黄瓜抗黑斑病基因连锁的SCAR标记及抗病资源筛选[J].华北农学报,2014,(1):208-212,5.基金项目
天津市自然科学基金项目(11JCYBJC09000) （11JCYBJC09000）
