河北医学Issue(3)：353-355,3.DOI:10.3969/j.issn.1006-6233.2014.03.001
HPLC 法测定兔血浆中阿西美辛和吲哚美辛的浓度
Determination of Acemetacin and Indometacin in Rabbits Plasma by HPLC
摘要
Abstract
Objective:To establish a method for the determination of acemetacin and indometacin con-centrations in rabbits plasma .Method: HPLC method was adopted .Ketoprofen was used as the internal standard,Hypersil BDS C18 (4.6 mm×250mm,5μm) column was used,the mobile phase consisted pH4.5 acetate buffer solution-acetonitrile-methanol(50 ∶40 ∶10),flow rate was 0.6mL/min,column temperature was 25℃,the detection wave length was 250nm,injection volume was 20μL.Result: The linear range of acemetacin was 0.04-2.56μg/mL,the average recovery was 89.4%,RSD=5.2%(n=5);The linear range of indometacin was 0.03-1.92μg/mL,the average recovery was 89.7%,RSD=5.7%(n=5).Conclusion:The method is simple ,sensitive and accurate , and it can be applied to the determination of acemetacin and indo-metacin concentrations in rabbits plasma .关键词
阿西美辛/吲哚美辛/高效液相色谱法Key words
Acemetacin/Indometacin/HPLC引用本文复制引用
刘振华,金英,李忠思..HPLC 法测定兔血浆中阿西美辛和吲哚美辛的浓度[J].河北医学,2014,(3):353-355,3.基金项目
河北省科技厅指导性课题 （）
