河北医学Issue(6)：901-904,4.DOI:10.3969/j.issn.1006-6233.2014.06.008
玉屏风散中抗氧化成分的研究
Study on the Antioxidant Activity of Yupingfeng Powder
摘要
Abstract
Objective:Screening the antioxidant activeity of Yupingfeng power , which lays the founda-tion for the pharmacy and pharmacology research .Method:The water extraction of Yupingfeng powder in the crude polysaccharide extraction process .According to optimal alcohol precipitation method ,using Sevag meth-od of removing protein was purified polysaccharide , another with ethanol reflux extraction powder by rotary e-vaporator steamed to obtain alcohol extraction of concentrated extract , and then were dissolved in deionized water withfour different the polarity of the solvent extraction , concentrate on four different polar parts , to ex-tract polysaccharide and four kinds of different polar solvents were used to the adjacent benzene three phenol -ic autoxidation method were used to determine the antioxidant activity .Result:The clearance rate of superox-ide anion radical n-butanol layer has higher , ethyl acetate layer clearance times , Yupingfeng powder apron to superoxide anion radical scavenging rate was higher than that of liquid petroleum ether and dichlorometh -ane layer.Conclusion:The mixture of Yupingfeng Powder with superoxide anion free radical scavenging a-bility of the active substance , its polarity may be relatively large , easily by n-butanol and ethyl acetate ex-traction, and seldom be non-polar petroleum ether and dichloromethane extraction amount .关键词
抗氧化活性/醇提浸膏/总多糖/水提醇沉Key words
Antioxidant activity/Ethanol extraction/Total polysaccharide/Water extraction and alcohol precipitation引用本文复制引用
袁明勇,张仲林,王丹,赵霞,廖昌军,郑玲利..玉屏风散中抗氧化成分的研究[J].河北医学,2014,(6):901-904,4.基金项目
四川省科技厅应用基础项目，编号2012JY0035 （）
