郑州大学学报（医学版）Issue(4)：440-443,4.DOI:10.3969j/.issn.1671-6825.2013.04.002
EC9706细胞和人食管鳞状细胞癌组织中 MAL 基因启动子区的甲基化状态
Methylation analysis of MAL gene promoter region in EC9706 cell and human esophageal squamous cell carcinoma tissue
刘伯新 1郭长青 1王曼华 1王明荣2
作者信息
- 1. 郑州大学第一附属医院消化内科郑州450052
- 2. 中国医学科学院北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室北京100021
- 折叠
摘要
Abstract
Aim:To explore methylation status of MAL gene promoter region in human esophageal cancer .Methods:Bisulfite-sequencing was used to detect the methylation pattern of MAL gene promoter region in esophageal cancer cell line EC9706 .Then the methylation pattern of MAL gene promoter region in esophageal cancer and matched para -cancerous tissue was further detected by methylation sensitive restriction endonuclease which was selected according to the sequencing results and PCR.Results:A total of 42 cytosine locuses were detected methylated in MAL gene promoter region in EC 9706,ac-counting for 73.7%(42/57) of all CpG locuses.Methylation incidence of MAL gene in esophageal cancer was 53.8%(14/26),much higher than 7.7%(2/26) in matched para-cancerous tissue (χ2 =10.924,P<0.001).Methylation of MAL gene promoter region in esophageal cancer with different clinicopathological features had no obvious significance ( P>0.05).Conclusion:The methylation of MAL gene promoter region may be one of the possible mechanisms of the occur-rence of esophageal cancer .关键词
食管鳞状细胞癌/EC9706细胞/MAL基因/启动子区甲基化Key words
esophageal squamous cell carcinoma/EC9706 cell/MAL gene/promoter region methylation分类
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刘伯新,郭长青,王曼华,王明荣..EC9706细胞和人食管鳞状细胞癌组织中 MAL 基因启动子区的甲基化状态[J].郑州大学学报（医学版）,2013,(4):440-443,4.
