郑州大学学报（医学版）Issue(4)：485-488,4.DOI:10.3969/j.issn.1671-6825.2013.04.014
PDTC对 A549细胞增殖、细胞周期、凋亡及星形细胞上调基因-1表达的影响
Effect of PDTC on proliferation,cell cycle,apoptosis and AEG-1 expres-sion of A549 cells
陈李影慧 1吴武佳 1秦东春1
作者信息
- 1. 郑州大学第一附属医院检验科郑州450052
- 折叠
摘要
Abstract
Aim:To explore the effect of pyrrolidine dithiocarbamate (PDTC) on proliferation,cell cycle,apoptosis of human lung cancer cell line A549 and expression of astrocyte elevated gene-1(AEG-1).Methods:A549 cells were treated by PDTC with different concentrations (25,50,100,200 μmol/L) for different time(24,48,72 h)respectively.MTT assay was used to detect the inhibition rates of cell growth .Flow cytometry was used to analyze the changes of cell cycle .Ho-echst33342 staining was used to observe the apoptosis of cells with the fluorescence microscope .RT-PCR and Western blot were used to detect the expression of AEG-1.Results:PDTC inhibited the A549 cell proliferation in a time-and dose-de-pendent manner(Ftime =531.981,Fconcentration =388.475,P<0.05).In contrast to the control, the percentage of cells in G0/G1 phase increased significantly in PDTC group (P<0.05)with apparent apoptotic morphological changes .The mRNA and protein expressions of AEG-1 were reduced with increasing concentration of PDTC (F=275.162 and 59.473,P<0.05).Conclusion:PDTC can inhibit the proliferation, and induce the apoptosis of A549 cells.The mechanism may be related to the down-regulated expressions of NF-κB and AEG-1 gene induced by PDTC .关键词
肺癌/A549细胞/PDTC/NF-κB/凋亡/星形细胞上调基因-1Key words
lung cancer/A549 cell/pyrrolidine dithiocarbamate/NF-κB/apoptosis/astrocyte elevated gene-1分类
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陈李影慧,吴武佳,秦东春..PDTC对 A549细胞增殖、细胞周期、凋亡及星形细胞上调基因-1表达的影响[J].郑州大学学报（医学版）,2013,(4):485-488,4.
