郑州大学学报（医学版）Issue(4)：493-496,4.DOI:10.3969/j.issn.1671-6825.2013.04.016
沉默 CTHRC1表达对乳癌 MDA-MB-157细胞体外侵袭转移能力的影响
Effect of silencing CTHRC1 on invasion and metastasis ability of breast cancer cell line MDA-MB-157 in vitro
梁冰 1王峰 2刘景超 1陈建中1
作者信息
- 1. 郑州大学第二附属医院乳腺外科郑州450014
- 2. 郑州大学第一附属医院肿瘤科郑州450052
- 折叠
摘要
Abstract
Aim:To explore the effect of silencing CTHRC1 by RNAi technique on the invasion and metastasis a-bility of breast cancer cell line MDA-MB-157 .Methods:Chemically synthesized siRNA targeting CTHRC1 gene was transfected into MDA-MB-157 cells at different concentrations (10, 20, 30 nmol/L) respectively, and MDA-MB-157 cells transfected with negative control siRNA and untransfected MDA-MB-157 cells were used as control.48 h af-ter transfection, RT-PCR and Western blot were used to detect the expressions of CTHRC1 mRNA and protein.The CTHRC1 siRNA with the highest interference efficiency was transfected into MDA-MB-157 cells again, and MDA-MB-157 cells transfected with negative control siRNA, or Lipofectamine 2000 and untransfected cells were used as con-trol.Boyden chamber was used to detect the invasion ability of MDA-MB-157 cells before transfection and 24, 48, 72 h after transfection.48 h after transfection, wound healing assay was used to detect the migration ability of MDA-MB-157 cells.Results: The expressions of CTHRC1 mRNA and protein among the groups were obviously different (F=28.185 and 17.776,P <0.001);with the increase of concentration of CTHRC1 siRNA, the expressions of CTHRC1 mRNA and protein decreased;with the 30 nmol/L CTHRC1 siRNA transfection, the interference efficiency reached the highest.After transfected with 30 nmol/L CTHRC1 siRNA, the numbers of MDA-MB-157 cells which traversed the membrane and the ability of wound healing decreased greatly(P<0.05).Conclusion: The invasion and metastasis ability of MDA-MB-157 cells could be decreased efficiently after inhibiting the expression of CTHRC1.关键词
胶原三股螺旋重复蛋白1/乳癌/RNA干扰/侵袭/转移Key words
collagen triple helic repeat containing 1/breast cancer/RNA interference/invasion/metastasis分类
医药卫生引用本文复制引用
梁冰,王峰,刘景超,陈建中..沉默 CTHRC1表达对乳癌 MDA-MB-157细胞体外侵袭转移能力的影响[J].郑州大学学报（医学版）,2013,(4):493-496,4.
