郑州大学学报（医学版）Issue(4)：544-547,4.DOI:10.3969/j.issn.1671-6825.2013.04.032
人肝脏组织 CYP3 A4酶活性的高效液相色谱法测定
Establishment of a HPLC method to detect CYP 3 A4 activity in human hepatic tissue
张海朋 1赵杰 1张莉蓉 2王晓飞 2薛文华1
作者信息
- 1. 郑州大学第一附属医院药学部郑州450052
- 2. 郑州大学基础医学院药理学教研室郑州450001
- 折叠
摘要
Abstract
Aim:To establish a HPLC method for determination of CYP 3A4 activity in human hepatic tissue .Meth-ods:Human liver microsome of 21 cases of liver cancer ,16 cases of hepatic hemangioma ,and 13 cases of gallbladder lesion was prepared for HPLC analysis .The analytical column was Angel C-18 column (4.6 mm ×250 mm, 5 μ), mobile phase consisted of methanol-acetonitrile-water( volume ratio =53:13:34 ) with flow rate at 1 mL/min, detective wave-length at 220 nm, and column temperature at 25℃,and the injection volume was 40μL..Results:1’-OH MDZ and MDZ retention time was 10.05 and 15.02 min with symmetrical peak and full separation (R>1.5) .The linear range of 1'-OH MDZ was 200~6 000 μg/L.Extraction recoveries at low , medium and high concentrations were all higher than 90%, re-spectively .The intra-day and inter-day precision RSD was less than 5%, and the stability RSD was less than 5%.The sta-tistical analysis showed that the liver diseases, gender or age had no significant effect on CYP3A4 activity(P>0.05). Conclusion:The HPLC method is rapid, stable with high repeatability.It is suitable for the determination of CYP3A4 ac-tivity in human liver .The liver diseases , gender and age have no significant effects on the Km.关键词
高效液相色谱/肝脏/CYP3A4Key words
high-performance liquid chromatography/liver/CYP3A4分类
医药卫生引用本文复制引用
张海朋,赵杰,张莉蓉,王晓飞,薛文华..人肝脏组织 CYP3 A4酶活性的高效液相色谱法测定[J].郑州大学学报（医学版）,2013,(4):544-547,4.
