郑州大学学报（医学版）Issue(6)：724-728,5.DOI:10.3969/j.issn.1671-6825.2013.06.003
半边旗提取物5F 对乳癌细胞 MDA-MB-231培养物诱导的HUVEC血管生成潜能的影响
Effect of Pteris semipinnata L extract 5F on angiogenic abilities of HU-VEC induced by the culture of MDA-MB-231 cells
摘要
Abstract
Aim:To investigate the effect and its possible mechanisms of Pteris semipinnata L extract(5F) on angio-genic abilities of human umbilical vein endothelial cells ( HUVEC) induced by the culture of human high metastatic breast cancer MDA-MB-231 cells.Methods:MTT assay was used to evaluate the cell proliferation of HUVEC induced by the cul-ture of MDA-MB-231 cells after being treated by 5F for 6 h and 24 h.The effect of 5F on invasion and migration of HUVEC induced by the culture of MDA-MB-231 cells was measured by transwell chamber assay .The adhesive potential of HUVEC cells induced by the culture of MDA-MB-231 cells was tested by cell-matrix adhesion assay .RT-PCR and Western blot were applied to detect the expression levels of KDR , Flt-1 mRNA and protein in HUVEC induced by the culture of MDA-MB-231 cells.Results:5F inhibited the proliferation of HUVEC induced by the culture of MDA-MB-231 cells after 6 h and 24 h treatment(Fgroup =64.852,Ftime =131.393,P<0.001).20, 40 and 80μmol/L 5F significantly inhibited the in-vasion, migration and adhesion to matrigel of HUVEC induced by the culture of MDA-MB-231 cells ( F =48.206, 147.613, 22.081,P<0.001).After 24 h treatment, 5F down-regulated the expressions of KDR, Flt-1 mRNA and protein (mRNA:F=86.381, 79.175;protein:F=36.621, 127.910,P<0.001) induced by the culture of MDA-MB-231 cells. Conclusion:5F could inhibit the proliferation , invasion, and migration of HUVEC induced by the culture of MDA-MB-231 cells and adhesion to matrix of HUVEC ,which may be involved in its down-regulation of the expression of KDR .关键词
半边旗提取物/5F/人脐静脉内皮细胞/KDR/Flt-1/血管生成/MDA-MB-231Key words
Pteris semipinnata L extract/5F/HUVEC/KDR/Flt-1/angiogenesis/MDA-MB-231分类
医药卫生引用本文复制引用
何振辉,翁闪凡,何太平,覃燕梅,梁念慈..半边旗提取物5F 对乳癌细胞 MDA-MB-231培养物诱导的HUVEC血管生成潜能的影响[J].郑州大学学报（医学版）,2013,(6):724-728,5.基金项目
国家自然科学基金资助项目39870900；广东省中医药局建设中医药强省课题20111057；佛山市医学类科技攻关项目201108064；佛山科学技术学院博士启动基金2012006 （）
