郑州大学学报（医学版）Issue(6)：753-757,5.DOI:10.3969/j.issn.1671-6825.2013.06.011
rhG-CSF对大鼠缺血缺氧神经元巨自噬水平的影响
Effects of rhG-CSF on macroautophagy of ischemia hypoxia SH-SY5Y cells and rat neurons
摘要
Abstract
Aim:To explore the effect and significance of rhG-CSF on neuronal macroautophagy in hypoxia ischemia rats.Methods:Cell hypoxia model was established by taking oxygen-glucose deprivation.After dealing cells with 0,100, 200 μg/L rhG-CSF, the marker of macrophage , LC3Ⅱ/LC 3Ⅰ was measured by Western blot , macroautophagy vesicle change was analyzed by monodansylcadaverine ( MDC) , and MTT was used to investigate the cell viability .Focal cerebral ischemia rat model was established by improved suture method .The modified neurological severity score (mNSS)was adopt-ed to evaluate neural function recovery before and after the intervention of rhG-CSF,LC3Ⅱ/LC3 Ⅰ of cortical neurons in rats was observed by Western blot , and LC3 protein expression was detected by immunohistochemistry .Results:With the increase of the dosage of rhG-CSF, the ratio of LC3Ⅱ/LC3Ⅰ and the number of macroautophagy vesicles gradually in-creased (F=288.686,154.698,P<0.001),and the cell viability also gradually increased (F=74.456, P<0.001). Compared with sham operation group and model group , LC3Ⅱ/LC3Ⅰand LC3-positive cells (F=573.085 and 88.945, P<0.001) raised in rhG-CSF group.After treatment, the mNSS of rats in rhG-CSF group was lower ( Fgroup =20.403, Ftime =19.193,Fmutual =33.415,P<0.001).Conclusion: rhG-CSF may participate in the neural protection after focal cerebral ischemia by raising macroautophagy activity .关键词
重组人粒细胞集落刺激因子/巨自噬/缺血缺氧/大鼠/SH-SY5YKey words
recombinant human granulocyte colony stimulating factor/macroautophagy/ischemia hypoxia/rat/SH-SY5Y分类
医药卫生引用本文复制引用
方立,王雪晶,荆婧,马耀华,邓文静,张雯雯,滕军放..rhG-CSF对大鼠缺血缺氧神经元巨自噬水平的影响[J].郑州大学学报（医学版）,2013,(6):753-757,5.基金项目
河南省基础与前沿技术研究计划项目092102310217；河南省医学科技攻关项目200903037 （）
