郑州大学学报（医学版）Issue(6)：799-802,4.DOI:10.3969/j.issn.1671-6825.2013.06.024
益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响
Effects of Compound Yifei Huoxue on cell microstructure and secretion function of human pulmonary artery endothelial cells
摘要
Abstract
Aim:To investigate the effects of Compound Yifei Huoxue ( CYH) on the cell microstructure and secretion function of human pulmonary artery endothelial cells .Methods:A model of hypoxia hypercapnia was established .Different doses of pharmacy (10,20,40 g/kg crude drug) were added into the culture medium to prepare serum containing CYH . After incubation for 12 h,the cell microstructure was observed by transmission electron microscopy , the levels of ET-1 and PGI2 were tested by radioimmunoassay method , and NO level was tested by nitrate reductase .Results:Hypoxic hypercap-nia caused damage to the cell microstructure ,while moderate and high dose CYH could alleviate the damage .There were significant differences in the levels of ET-1, NO and PGI2 among 3 CYH model group and blank control group ( F =121.870, 60.040, 49.060,P<0.001).The level of ET-1 in the model group was higher than that of blank control group , but the levels of NO and PGI2 were lower(P<0.01).The levels of ET-1 in moderate and high dose CYH groups were low-er than that of model group,but the levels of NO and PGI2 were higher(P<0.01).Conclusion: Hypoxia hypercapnia could cause human pulmonary artery endothelial cell microstructure damage and dysfunction .Moderate and high dose CYH could alleviate the damage .关键词
益肺活血复方/低氧高二氧化碳/人肺动脉内皮细胞/细胞微结构/内皮素-1/一氧化氮/前列环素Key words
Compound Yifei Huoxue/hypoxia hypercapnia/human pulmonary artery endothelial cell/cell micro-structure/endothelin-1/nitric oxide/prostacyclin分类
医药卫生引用本文复制引用
王婷,欧敏,黄友章..益肺活血复方对人肺动脉内皮细胞微结构及内分泌功能的影响[J].郑州大学学报（医学版）,2013,(6):799-802,4.基金项目
全军医药卫生科研基金资助项目36040 （）
