郑州大学学报（医学版）Issue(1)：4-7,8,5.DOI:10.3969/j.issn.1671-6825.2014.01.002
沉默 GST-π基因表达对 EC9706／cDDP 细胞顺铂耐药性的影响
Effect of GST-πexpression and mechanisms of reversing drug-resistant to EC9706/cDDP of esophageal cancer cells
唐悦 1轩小燕 1柳璐璐 1李敏1
作者信息
- 1. 郑州大学基础医学院微生物学与免疫学教研室郑州450001
- 折叠
摘要
Abstract
Aim:To observe the effects of silencing the expression of GST-πon the drug-resistance of esophageal car-cinoma cells.Methods:The lentivirus of siRNA targeting to GST-πwas constructed.EC9706/cDDP cells were infected by the lentivirus named GSTsi1,GSTsi2.The mRNA and protein expressions of GST-πbefore and after infection were measured by RT-PCR and Western blot.Cells were divided into four groups:cells infected with GSTsi2, GSTsiC, untreat-ed EC9706/cDDP and EC9706 .Drug sensitivity to cDDP was determined by MTT .Results:The expression level of GST-πmRNA in cells infected by GSTsi1 and GSTsi2 were lower than those in control cells (F=3.490,P<0.001),and the ex-pression of GST-πprotein was also decreased .Compared with untreated EC9706/cDDP and EC9706/cDDP infected by GSTsiC,the resistance index of EC9706/cDDP infected by GSTsi2 decreased(F=50.510,P<0.001).Conclusion: Si-lencing the expression of GST-πby RNAi can partly reverse the drug resistance of EC 9706/cDDP.关键词
EC9706/cDDP细胞/耐药/谷胱甘肽S转移酶-π/RNA干扰Key words
EC9706/cDDP cell/drug-resistant/glutathione S-transferase-π/RNA interfering分类
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唐悦,轩小燕,柳璐璐,李敏..沉默 GST-π基因表达对 EC9706／cDDP 细胞顺铂耐药性的影响[J].郑州大学学报（医学版）,2014,(1):4-7,8,5.
