郑州大学学报（医学版）Issue(1)：8-11,4.DOI:10.3969/j.issn.1671-6825.2014.01.003
冬凌草甲素对 EC9706细胞增殖、凋亡的影响
Influence of oridonin on EC9706 cell proliferation and apoptosis
摘要
Abstract
Aim:To study the influence of oridonin on esophageal squamous cell carcinoma EC 9706 cell proliferation and apoptosis in vitro .Methods:MTT assay was used to detect the inhibition effect of oridonin on the growth of EC 9706;flow cytometry was applied to detect the impact of oridonin on cell cycle and apoptosis of EC 9706 , and calculated the apop-tosis rate of cells;change of Ca 2+fluorescence intensity of EC 9706 affected by different concentrations of oridonin was ob-served by laser scanning confocal microscope .Results:MTT assay showed that the inhibition rate in the experiment group increased with the rising of concentration (F=370.600,P<0.001) after oridonin acting on EC9706; results of cell cycle detected by flow cytometry showed that the number of cell in G 0 and G1 stage increased notably after oridonin acting on EC9706 for 48 hour respectively(F=24.200,P<0.001);results of apoptosis showed that oridonin could obviously induce the apoptosis of EC9706(P <0.05), and the effect was the best when the oridonin concentration was 40 μmol/L(F =10.214,37.820,P <0.001).Different concentrations of oridonin could increase Ca 2 +fluorescence intensity of EC9706,and the difference was significant (F =24.400,P <0.001).Conclusion: oridonin can improve the inhibition effects onproliferation and apoptosis of EC9706 cell.关键词
冬凌草甲素/食管鳞状细胞癌/EC9706/增殖/凋亡Key words
oridonin/esophageal squamous cell carcinoma/EC9706/proliferation/apoptosis分类
医药卫生引用本文复制引用
刘俊保,岳静宇,唐引引..冬凌草甲素对 EC9706细胞增殖、凋亡的影响[J].郑州大学学报（医学版）,2014,(1):8-11,4.基金项目
河南省卫生厅5451项目2011077 （）
