郑州大学学报（医学版）Issue(1)：11-14,4.DOI:10.3969/j.issn.1671-6825.2014.01.004
冻存对细胞因子诱导的杀伤细胞免疫表型及细胞内因子表达的影响
Effects of cryopreservation on CIK immunophenotype and intracellular cy-tokine expression
摘要
Abstract
Aim: To explore the effects of cryopreservation on cytokine-induced killer cell ( CIK ) immunophenotype and intracellular cytokine expression .Methods:Peripheral blood mononuclear cells from ten patients with cancer were iso-lated by Ficoll-Plaque density gradients and CIKs were obtained by incubating with appropriate cytokines .The secretion levels of perforin, granzyme-B and interferon-γin CIK cryopreserved for 3 months and recovered for 4,24,and 72 h were detected by flow cytometry assay and intracellular staining , and cellular activity were detected by PI and AnnexinV stai-ning.The fresh CIKs were the control .Results:There was no significant difference in the levels of granzyme-B or interfer-on-γbetween fresh CIK and those recovered for 4 h(t=1.029,0.237,P>0.05).However, the level of perforin in fresh CIK, those recovered for 4,24,and 72 h cells were (35.97 ±7.12)%,(10.00 ±6.04)%,(17.60 ±3.92)% and (35.20 ±6.23)%, and there was significant difference (F=47.480,P<0.001);the expression level in perforin cryopre-served CIK decreased and gradually reached to the normal level with time .Cellular activity of CIK also recovered gradually (P<0.05).Conclusion:Cryopreservation might change the cell subtypes of CIK .关键词
细胞因子诱导的杀伤细胞/细胞内因子/冻存/免疫表型Key words
cytokine-induced killer cell/intracellular cytokine/cryopreservation/immunophenotype分类
医药卫生引用本文复制引用
王菲,王丽萍,张震,岳冬丽,段秀芳,张斌,张毅..冻存对细胞因子诱导的杀伤细胞免疫表型及细胞内因子表达的影响[J].郑州大学学报（医学版）,2014,(1):11-14,4.基金项目
国家自然科学基金资助项目81171986，81271815；卫生部科研攻关基金项目20110110001；河南省科技厅基础与前沿技术研究基金项目112300410153，122300410155；河南省科技厅科技创新人才计划项目124200510006；郑州大学第一附属医院院内创新团队基金项目 （）
