郑州大学学报（医学版）Issue(1)：102-105,4.DOI:10.3969/j.issn.1671-6825.2014.01.029
BMP-2基因转染对兔骨髓间充质干细胞碱性磷酸酶活性和钙含量的影响
Effect of BMP-2 gene transfection on alkaline phosphalase activity and calcium deposition in bone marrow mesenchymal stem cell of rabbits
张雁儒 1张辉 2马辉 3王义生4
作者信息
- 1. 郑州大学国际教育学院医学实验中心郑州450001
- 2. 郑州大学第一附属医院骨科郑州450052
- 3. 郑州大学临床医学系郑州450052
- 4. 洛阳市第三人民医院骨科471002
- 折叠
摘要
Abstract
Aim:To investigate the changes of alkaline phosphalase ( ALP) activity and calcium deposition of rabbit bone marrow mesenchymal stem cells (BMSCs) transfected with BMP-2 gene.Methods:The bone marrow were extracted from posterior superior iliac spine of 16 adult rabbits ,and mixed to be cultured .BMSCs were obtained and transfected with pcDNA3.1-BMP-2.At the 7th,14th,21st,28th and 35th day during the amplification culture ,ALP activity and calcium dep-osition were detected;at the 21st day, BMP-2 mRNA was detected using RT-PCR.The BMSCs without transfection were the control.Results:The expression of BMP-2 mRNA was (1.652 ±0.082) in transfected BMSCs, and (0.473 ±0.021) in the control BMSCs,and the difference between them was significant (t=2.192,P=0.005).ALP activity and calcium deposition of transfected cells were higher than those of the control (P<0.01).Conclusion:BMSCs transfected with BMP-2 gene show obvious osteogenic activity ,and could be used as seed cells for bone engineering research .关键词
骨形成蛋白2/骨髓间充质干细胞/碱性磷酸酶/钙/兔Key words
bone morphogenetic protein 2/bone marrow mesenchymal stem cell/alkaline phosphalase/calcium/rabbit分类
医药卫生引用本文复制引用
张雁儒,张辉,马辉,王义生..BMP-2基因转染对兔骨髓间充质干细胞碱性磷酸酶活性和钙含量的影响[J].郑州大学学报（医学版）,2014,(1):102-105,4.
