郑州大学学报（医学版）Issue(2)：157-161,5.DOI:10.13705/j.issn.1671-6825.2014.02.005
Matrigel胶对大鼠海马神经干细胞增殖及分化的影响
Effect of Matrigel on the proliferation and differentiation of rat hippocam-pal neural stem cells
摘要
Abstract
Aim:To observe the effect of Matrigel on the proliferation and differentiation of rat hippocampus neural stem cells(NSCs).Methods:NSCs were extracted from newborn SD rats′hippocampus, and the cells after 3 generations were identified through Nestin cytochemical staining .The NSCs were allocated into four groups:group A was inoculated on 24-well plate coated by poly-lysine and added normal culture medium;group B was inoculated on 24-well plate coated by poly-lysine and added DMEM/F12 medium containing fetal bovine serum; group C was inoculated on 24-well plate coated by Matrigel and added normal culture medium;group D was inoculated 24-well plate coated by Matrigel and add-ed DMEM/F12 medium contarning fetal bovine serum .After a week, the expressions of Nestin ,β-tubulinⅢand glial fibril-lary acidic protein(GFAP) by immunofluorescence staining.Results:Nestin protein was positive in the cells after 3 genera-tions, which confirmed that they were NSCs .NSCs in group A differentiated slowly , while group B,C and D differentiated rapidly.Especially, NSCs in group C and D formed three-dimensional network structure .The immunofluorescence staining positive rate of Nestin,β-tubulinⅢ and GFAP among the four groups had statistical significance ( Fmedium =3 918.000, 437.023, and 3 395.000 respectively,Fcoat =291.234,4 833.000, and 50.081 respectively,P<0.001),which showed that NSCs in group B,C and D were significantly differentiated , and the trend of NSCs differentiated into neurons was more obvi-ous in group C,D,especially group C.Conclusion:Matrigel could induce NSCs to differentiate into neurons .关键词
神经干细胞/Matrigel胶/巢蛋白/β-tubulinⅢ/胶质纤维酸性蛋白/大鼠Key words
neural stem cell/Matrigel/Nestin/β-tubulinⅢ/glial fibrillary acidic protein/rat分类
生物科学引用本文复制引用
邓鑫,宋来君,郭新宾..Matrigel胶对大鼠海马神经干细胞增殖及分化的影响[J].郑州大学学报（医学版）,2014,(2):157-161,5.基金项目
国家自然科学基金资助项目340600531547 （）
