郑州大学学报（医学版）Issue(2)：166-168,3.DOI:10.13705/j.issn.1671-6825.2014.02.007
不同刺激物对细胞因子诱导的杀伤细胞分泌细胞因子的影响
Influence of different stimulators on secretion of cytokines by cytokine-in-duced killer cells
摘要
Abstract
Aim:To investigate the effects of anti-CD3 mAb and different concentrations of anti-CD28 mAb stimuli on the secretion of cytokines by cytokine-induced killer(CIK) cells.Methods:CIK cells were obtained from peripheral blood of 6 tumor patients,and stimulated by 1.0 mg/L anti-CD3 mAb combined with 0.5,1.0 or 2.5 mg/L anti-CD28 mAb,re-spectively.The secretion of IFN-γ, IL-10, IL-2 and TNF-αwas measured by ELISA.Flow cytometry was used to detect the expressions of IFN-γand perforin in CIK cells .CIK cells stimulated by 50 μg/L PMA combined with 750 μg/L iono-mycin were the control.Results:There were no statistical differences in the secretion levels of IL-10, IL-2, IFN-γ, TNF-αor the expressions of IFN-γand perforin in CIK cells among the different stimulatory concentrations of anti-CD3 mAb/an-ti-CD28 mAb groups(P>0.05).Conclusion:There is no obvious difference in cytokine secretory capacity after stimula-tion with different concentrations of anti-CD3 mAb/anti-CD28 mAb.Thus 1.0 mg/L anti-CD3 mAb and 0.5 mg/L anti-CD28 mAb may be chosen to stimulate lymphocytes .关键词
抗CD3单抗/抗CD28单抗/细胞因子/细胞因子诱导的杀伤细胞Key words
anti-CD3 mAb/anti-CD28 mAb/cytokine/cytokine-induced killer cell分类
医药卫生引用本文复制引用
李静,王菲,刘艳芬,王丽萍,许钰杰,王盟,张斌,张毅..不同刺激物对细胞因子诱导的杀伤细胞分泌细胞因子的影响[J].郑州大学学报（医学版）,2014,(2):166-168,3.基金项目
国家自然科学基金资助项目81171986，81271815；卫生部科研攻关基金项目20110110001；河南省科技厅基础与前沿技术研究基金112300410153，122300410155；河南省科技厅科技创新人才计划项目124200510006；郑州大学第一附属医院院内创新团队基金项目 （）
