郑州大学学报（医学版）Issue(2)：169-172,4.DOI:10.13705/j.issn.1671-6825.2014.02.008
苦参碱对人鼻咽癌CNE2细胞裸鼠移植瘤生长及凋亡相关基因表达的影响
Effect of matrine on growth and apoptosis-related genes expressions of human nasopharyngeal carcinoma CNE 2 cell xenografts in nude mice
摘要
Abstract
Aim:To investigate the effect of matrine on expressions of apoptosis-related genes and growth of human na-sopharyngeal carcinoma CNE 2 cell xenografts in nude mice .Methods:A total of 20 nude mice were subcutaneously inocu-lated with CNE2.After the successful establishment of xenograft model ,the 20 nude mice were randomly divided into 4 groups, 5 rats in each group .Low-and high-dose matrine groups were intraperitoneally injected with matrine at dose of 50 and 100 mg/kg, positive control group was injected with cisplatin ,and negative control group received normal saline .After 7 times administration , the tumor inhibition rate was detected , and the mRNA and protein expressions of bax , bcl-2 and caspase-3 were detected by real-time PCR and Western blot,respectively.Results: The tumor inhibition rates of positive control group, and low-,high-dose matrine groups were 51.6%, 26.4%, and 43.2%;the mRNA and protein expressions of bax,bcl-2 and caspase-3 in xenograft tissue had significant differences (F=3.751,16.113,3.918 and 26.500,9.771, 12.255, P<0.05).Compared with the negative control group , the mRNA and protein expressions of bax in positive con-trol group and low-,high-dose matrine groups and those of caspase-3 in positive control group and high-dose matrine group increased(P<0.05),but those of bcl-2 decreased(P<0.05).Conclusion: Matrine could inhibit the growth of CNE2 xenograft through regulating the expressions of apoptosis-related genes .关键词
苦参碱/鼻咽癌/凋亡/移植瘤/裸鼠Key words
matrine/nasopharyngeal carcinoma/apoptosis/xenograft/nude mice分类
医药卫生引用本文复制引用
何光耀,谢貌,唐安洲,谭颂华..苦参碱对人鼻咽癌CNE2细胞裸鼠移植瘤生长及凋亡相关基因表达的影响[J].郑州大学学报（医学版）,2014,(2):169-172,4.基金项目
国家自然科学基金资助项目81241113；广西教育厅科研立项项目201106LX088 （）
