郑州大学学报（医学版）Issue(2)：203-206,4.DOI:10.13705/j.issn.1671-6825.2014.02.017
常压氧疗对大鼠脑缺血再灌注后脑组织 Caspase-9及 Caspase-3 mRNA表达的影响
Influence of normobaric oxygen on Caspase-9 and Caspase-3 mRNA ex-pression in brain tissue of cerebral ischemia-reperfusion rats
摘要
Abstract
Aim:To explore the effects of different concentrations of normobaric oxygen ( NBO ) on Caspase-9 and Caspase-3 mRNA expression in brain tissue of rats after acute cerebral ischemia-reperfusion .Methods:A total of 120 SPF level male SD rats were subjected to middle cerebral artery occlusion-reperfusion model .The rats were randomly divided in-to four groups , of which 3 groups ( n=90 ) were given three concentrations of NBO treatment respectively and the other group was the air group (n=30).When taking persistent oxygen or air for 3, 6, 12, 24, 48, 72 h, five rats randomly taken in each group would be sacrificed , with brain collected .Fluorescence quantitative PCR was used to detect Caspase-9 and Caspase-3 mRNA expression levels .Results:The expression of Caspase-9 and Caspase-3 mRNA peaked in air group and low, medium, high concentration of NBO treatment groups at 48 h, then began to decline(P<0.05), and at each time point those of the NBO groups were lower than that of air group (P<0.05).Conclusion:NBO could reduce Caspase-9 and Caspase-3 mRNA expression in cerebral ischemia-reperfusion.关键词
常压氧/脑缺血再灌注/Caspase-9/Caspase-3/凋亡/大鼠Key words
normobaric oxygen/cerebral ischemia-reperfusion/Caspase-9/Caspase-3/apoptosis/rat分类
医药卫生引用本文复制引用
陈素艳,张振香,高峰,张迎娜,王鹏,王云璐,王岚,高冰..常压氧疗对大鼠脑缺血再灌注后脑组织 Caspase-9及 Caspase-3 mRNA表达的影响[J].郑州大学学报（医学版）,2014,(2):203-206,4.基金项目
河南省科技厅基础与前沿技术研究计划基金资助项目122300410082；河南省教育厅5451基金资助项目201201065；郑州大学研究生创新基金资助项目12Y03701 （）
