化工进展Issue(12)：2946-2951,6.DOI:10.3969/j.issn.1000-6613.2013.12.026
响应面法优化壳聚糖酶产生菌Mitsuariasp.K1的产酶发酵条件
Optimization of fermentation conditions of Mitsuaria sp.K1 for chitosanase production by response surface methodology
摘要
Abstract
Nowadays,people pay much attention to chitosan and its degradation product due to its excellent physical characteristic and biological activity. In this study,a high Chitosanase-producing bacterium Mitsuaria sp.K1 was isolated from the soil of Qingdao seacoast by primary screening of plate specula and shaking cultivation. The optimization of fermentation conditions was studied by Response Surface Methodology. The results showed that,under the optimal composition of medium (1.0%powder chitosan,0.5%KNO3,0.22%KH2PO4,0.1%Na2HPO4,0.15%KCl,0.05%MgSO4·7H2O) and the optimal cultural condition(25.2 ℃ incubating temperature,25.4 hours incubating time,initial pH6.5,3%inoculation amount,100 mL medium/500 mL flask,160 r/min),the maximal chitosanase activity of Mitsuaria sp.K1 could reach 11.56 U/mL which was 5.32 times of primary strain. In addition,due to Mitsuaria sp.K1 with lower temperature and shorter fermentation time of chitosanase production,it has a good potential in industrial fermentation.关键词
壳聚糖酶/Mitsuaria sp.K1/发酵条件/响应面法/优化Key words
chitosanase/Mitsuaria sp.K1/fermentation condition/response surface methodology/optimization分类
化学化工引用本文复制引用
刘杰,王延鹏,许晶,王能飞..响应面法优化壳聚糖酶产生菌Mitsuariasp.K1的产酶发酵条件[J].化工进展,2013,(12):2946-2951,6.基金项目
“十二五”农村领域国家科技计划（2011BAD14B00）、国家海洋局海洋重点实验室开放基金（MBSMAT-2010-05）及国家科技基础条件平台建设项目（2005DKA21209）。 （）
