黑龙江八一农垦大学学报Issue(6)：17-22,42,7.
牛粪堆肥高温期氨氧化古菌与氨氧化细菌的多样性分析
Diversity of Ammonia-Oxidizing Bacteria and Ammonia-Oxidizing Archaea at High Temperature Phase during the Livestock Manure Composting Process
摘要
Abstract
The transmutation and loss of nitrogen materials were effected significantly by ammonia-oxidizing microorganisms during the composting process. In order to detect the diversity of Ammonia-Oxidizing Archaea (AOA)and Ammonia-Oxidizing Bacteria (AOB),the monooxygenase gene (amoA)was used to analyze as the tag at high-temperature phase during the livestock manure composting process. The results showed that Nitrosomonas genus and Nitrosospira genus were the dominant genus of AOB,and the amount of clones accounted for 59.3% and 40.7% among the clone library at the high-temperature period,respectively,and the amount of Nitrosomonas was more dominant than that of the Nitrosospira. For communities of AOA,the amount of AOA resulting from soil were greatly more than that of AOA resulting from sea. The ratio of clones of soil AOA was 94.2%,but the ratio of sea AOA accounted for only 5.8%among all of AOA clones.关键词
堆肥/amoA基因/氨氧化古菌(AOA)/氨氧化细菌(AOB)Key words
compost/gene amoA/Ammonia-Oxidizing Archaea(AOA)/Ammonia-Oxidizing Bacteria(AOB)分类
农业科技引用本文复制引用
孙志远,晏磊,王彦杰,林匡飞,李辉,王伟东..牛粪堆肥高温期氨氧化古菌与氨氧化细菌的多样性分析[J].黑龙江八一农垦大学学报,2013,(6):17-22,42,7.基金项目
国家自然科学基金项目（31270536）；国家科技支撑计划项目（2012BAD12B05）；黑龙江省高校科技创新团队项目（2012TD006）；黑龙江省普通高等学校新世纪优秀人才培养计划（1153-NCET-003）；黑龙江农垦总局“十二五”重大科技专项（HNK11A-10-01）。 （）
