黑龙江八一农垦大学学报Issue(6)：35-38,42,5.
牛传染性鼻气管炎的分离鉴定和灭活条件的优化
Isolation and identification of infectious bovine rhinotracheitis proliferation and inactivated Optimization
梁宏儒 1朱战波 1高佳滨 1李安 1陈为宏 1乔波 1尹辉 1胡旭 1赵达 1姜东君1
作者信息
- 1. 黑龙江八一农垦大学动物科技学院，大庆 163319
- 折叠
摘要
Abstract
A virus isolated from bovine nasal swab suspected infectious bovine rhinotracheitis virus infection by PCR and the MDBK cell lesions observed to identify it. The isolates strains of infectious bovine rhinotracheitis. Optimization of the strain proliferation conditions and formaldehyde inactivated conditions,to provide a reference for the preparation of infectious bovine rhinotracheitis cell-derived inactivated vaccine. The results show that the strain inoculation 18 h diseased cells to fight 70% began to collect the drugs,the virus was inactivated in 0.2%formaldehyde 37℃for 36 h inactivated the best.关键词
牛传染性鼻气管炎病毒/PCR/分离鉴定/甲醛灭活Key words
Infectious bovine rhinotracheitis virus/PCR/Isolation and identification/Inactivated condition分类
农业科技引用本文复制引用
梁宏儒,朱战波,高佳滨,李安,陈为宏,乔波,尹辉,胡旭,赵达,姜东君..牛传染性鼻气管炎的分离鉴定和灭活条件的优化[J].黑龙江八一农垦大学学报,2013,(6):35-38,42,5.
