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青岛老鹳草花粉和花芽扫描电镜制样条件探讨

胡春辉 卢婉佩 刘鹏起 刘庆华 袁玉清

黑龙江农业科学Issue(12):82-84,85,4.
黑龙江农业科学Issue(12):82-84,85,4.

青岛老鹳草花粉和花芽扫描电镜制样条件探讨

Study on Sampling Methods of Scanning Electron Microscope on Pollen and Bud of Geranium tsingtauense Yabe

胡春辉 1卢婉佩 1刘鹏起 1刘庆华 1袁玉清1

作者信息

  • 1. 青岛农业大学中心实验室,山东青岛266109
  • 折叠

摘要

Abstract

In order to explore the revelant types of Geranium pollen and flower buds preparation methods of scanning electron microscope ,air-dried and glutaric aldehyde fixed methods were compared to study preparation condition of scanning electron microscopy .The results showed that Geranium pollen grains prepared through direct observation were full and clear in the scanning electron microscopy ,microscopic structure were clear and morphological integrity ;micro-structure of flower bud treated with FAA single fixation were morphological in-tegrity ,layers rich ,stereo sense strong ,cell dehydration shrinkage was improved significantly .Through the comparison of different specimen preparation methods ,reliable technique which was easier in operation and low cost was achieved ,it had important guiding significance for the preparation of Geranium tsingtauense .pollen and bud .

关键词

冷场发射扫描电镜/青岛老鹳草/花粉/花芽/样品制备

Key words

scanning electron microscope (SEM )/Geranium tsingtauense Yabe./pollen/flower bud/specimen preparation

分类

生物科学

引用本文复制引用

胡春辉,卢婉佩,刘鹏起,刘庆华,袁玉清..青岛老鹳草花粉和花芽扫描电镜制样条件探讨[J].黑龙江农业科学,2013,(12):82-84,85,4.

基金项目

青岛农业大学实验技术研究资助项目 ()

黑龙江农业科学

1002-2767

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