黑龙江农业科学Issue(1)：7-10,11,5.
水稻基因组DNA提取方法的研究
Study on Extraction Method of Rice Genomic DNA
摘要
Abstract
In order to study fast and effective extraction method of rice genomic DNA for molecular marker-as-sisted breeding ,taking rice fresh leaves ,senescing leaves and rice seeds as experimental materials and CTAB and SDS as methods ,DNA purity was tested by UV spectrophotometer and agarose gel electrophoresis ,extrac-tion methods and experimental system of rice genomic DNA were optimized and explored .The results showed that quality extracted from fresh leaves was the best followed by seeds and senescing leaves was the last ;the difference between CTAB and SDS methods was not very obvious ,andβ-mercaptoethanol could added into so-lution to avoide the pollution of phenols oxidation .关键词
水稻/基因组DNA/CTAB法/SDS法/分子标记辅助育种Key words
rice/genomic DNA/CTAB/SDS/molecular marker-assisted breeding分类
农业科技引用本文复制引用
马文东..水稻基因组DNA提取方法的研究[J].黑龙江农业科学,2014,(1):7-10,11,5.基金项目
国家“十二五”科技支撑计划资助项目 （）
