黑龙江农业科学Issue(2)：62-64,3.
静置培养BHK-21细胞生长及代谢动力学研究
Growth and Metabolism Kinetic Research of BHK-21 Cells in Stationary Culture
摘要
Abstract
In order to research the grow th and metabolism kinetic characteristics of BHK-21 cells ,low cell densi-ty cultured method was used to determine cell density and vitality every day .The contents of Gluc ,Lac ,Gln , and NH4 + in medium were determined by multi-parameter biochemical analyzer ,and the specific metabolic rate were calculated .The results showed that the growth curve of BHK-21 cells was “S” type ,the largest density was 50 .3 × 104 upf·mL-1 and the doubling time was 21 .1 h .Specific consumption rate of Gluc and Gln were-3 .79 mg·(106 cells)-1 ·d-1 and -9 .95μmol·(106 cells)-1 ·d-1 in logarithmic phase ,and specific generation rate of Lac and NH4 + were 2 .81 mg·(106 cells)-1·d-1 and 6 .30 μmol·(106 cells)-1·d-1 .关键词
BHK-21细胞/生长特征/代谢动力学/比代谢速率/比消耗速率/比生成速率Key words
BHK-21 cell/growth characteristics/metabolism kinetics/specific metabolic rate/specific consump-tion rate/specific generation rate分类
生物科学引用本文复制引用
付智财,王家敏,沈武玲,令世鑫,马忠仁,乔自林..静置培养BHK-21细胞生长及代谢动力学研究[J].黑龙江农业科学,2014,(2):62-64,3.基金项目
甘肃省科技重大专项资助项目（1203FKDA030）；西北民族大学中央高校基金资助项目 （）
