哈尔滨商业大学学报(自然科学版)Issue(1):18-20,24,4.
鸡冠花乙醇提取物的 HPLC 指纹图谱研究
Study on HPLC fingerprint of Celosia cristata L.ethanol extract
张海晶 1金兰 1于金平 2陈大忠1
作者信息
- 1. 黑龙江中医药大学中医药研究院,哈尔滨150040
- 2. 东北农业大学食品学院,哈尔滨150030
- 折叠
摘要
Abstract
HPLC fingerprint of Celosia cristata L .was established to provide a reliable meth-od for the science appraisal and active control its quality .The chromatographic conditions were as follows:Dikma C18 chromatobar column (250 mm ×4.6 mm, 5 μm), gradient elu-tion with acetonitrile -0.1% phosphoric acid solution , volume flow rate of 1.0 mL/min, under the detection wave length of 257 nm, and record time of 90 min.Data was analyzed to compare the similarity of the samples for identifying the main chromatographic peaks further-more .The chromatographic fingerprint of 10 batches of Celosia cristata L .showed 16 charac-teristic peaks, with the similarity above 0.9.The qualities of Celosia cristata L .in different batches were in a good similarity .The method can be applied to quality of Celosia cristata L .关键词
鸡冠花/指纹图谱/高效液相色谱法Key words
Celosia cristata L ./fingerprint spectrum/HPLC分类
医药卫生引用本文复制引用
张海晶,金兰,于金平,陈大忠..鸡冠花乙醇提取物的 HPLC 指纹图谱研究[J].哈尔滨商业大学学报(自然科学版),2014,(1):18-20,24,4.