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鸡冠花乙醇提取物的 HPLC 指纹图谱研究

张海晶 金兰 于金平 陈大忠

哈尔滨商业大学学报(自然科学版)Issue(1):18-20,24,4.
哈尔滨商业大学学报(自然科学版)Issue(1):18-20,24,4.

鸡冠花乙醇提取物的 HPLC 指纹图谱研究

Study on HPLC fingerprint of Celosia cristata L.ethanol extract

张海晶 1金兰 1于金平 2陈大忠1

作者信息

  • 1. 黑龙江中医药大学中医药研究院,哈尔滨150040
  • 2. 东北农业大学食品学院,哈尔滨150030
  • 折叠

摘要

Abstract

HPLC fingerprint of Celosia cristata L .was established to provide a reliable meth-od for the science appraisal and active control its quality .The chromatographic conditions were as follows:Dikma C18 chromatobar column (250 mm ×4.6 mm, 5 μm), gradient elu-tion with acetonitrile -0.1% phosphoric acid solution , volume flow rate of 1.0 mL/min, under the detection wave length of 257 nm, and record time of 90 min.Data was analyzed to compare the similarity of the samples for identifying the main chromatographic peaks further-more .The chromatographic fingerprint of 10 batches of Celosia cristata L .showed 16 charac-teristic peaks, with the similarity above 0.9.The qualities of Celosia cristata L .in different batches were in a good similarity .The method can be applied to quality of Celosia cristata L .

关键词

鸡冠花/指纹图谱/高效液相色谱法

Key words

Celosia cristata L ./fingerprint spectrum/HPLC

分类

医药卫生

引用本文复制引用

张海晶,金兰,于金平,陈大忠..鸡冠花乙醇提取物的 HPLC 指纹图谱研究[J].哈尔滨商业大学学报(自然科学版),2014,(1):18-20,24,4.

哈尔滨商业大学学报(自然科学版)

1672-0946

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