华南农业大学学报Issue(4)：524-530,7.
偏肿革裥菌漆酶基因克隆及启动子序列分析
Cloning of Laccase Gene and Analysis of Its Promoter Sequence from Lenzites gibbosa
摘要
Abstract
The cDNA and Genomic DNA sequences of the laccase gene from Lenzites gibbosa were ob-tained by PCR and RACE technology , and the length of laccase gene was 2 165 bp.Comparison of the cDNA and DNA sequences showed that the laccase gene contained 11 exons and 10 introns.The cDNA length of laccase gene was 1 873 bp, including an ORF with 1 563 bp length and codes 520 amino acids . The closest organism was Trametes versicolor with 83%similarity level of amino acids .The 986 bp se-quences of promoter located in the upstream of start code of laccase gene were obtained by the approach of SEFA-PCR.The promoter not only scattered in the basic transcriptional elements of TATA-box, CAAT-box and AP2, but also contained seven elements of MRE , two elements of STRE, one element of HSEs and seven nitrogen factor binding sites , etc.The laccase gene expression of the Lenzites gibbosa can be regulated by different exterior inducers .关键词
偏肿革裥菌/漆酶基因/启动子克隆/转录调控Key words
Lenzites gibbosa/laccase gene/promoter cloning/transcriptional regulation分类
生物科学引用本文复制引用
郑苗苗,池玉杰..偏肿革裥菌漆酶基因克隆及启动子序列分析[J].华南农业大学学报,2013,(4):524-530,7.基金项目
国家自然科学基金（30671700）；中国博士后科学基金（20090460866）；齐齐哈尔市科技局社会发展攻关项目 （）
