偏肿革裥菌漆酶基因克隆及启动子序列分析OA北大核心CSCDCSTPCD
Cloning of Laccase Gene and Analysis of Its Promoter Sequence from Lenzites gibbosa
以优化后的漆酶培养基为基础，通过RT-PCR和RACE技术相结合，从偏肿革裥菌Lenzites gibbosa菌株中获得编码漆酶基因的cDNA及Genomic DNA的全长序列，Genomic DNA大小为2165 bp．通过比较该漆酶基因的cDNA和Genomic DNA的全长序列，发现该基因包含11个外显子和10个内含子．cDNA序列的全长为1873 bp，其中包含一个完整的ORF，长度为1563 bp，编码520个氨基酸．序列在氨基酸水平上…查看全部>>
The cDNA and Genomic DNA sequences of the laccase gene from Lenzites gibbosa were ob-tained by PCR and RACE technology , and the length of laccase gene was 2 165 bp.Comparison of the cDNA and DNA sequences showed that the laccase gene contained 11 exons and 10 introns.The cDNA length of laccase gene was 1 873 bp, including an ORF with 1 563 bp length and codes 520 amino acids . The closest organism was Trametes versicolor with 83%similarity level of amino ac…查看全部>>
郑苗苗;池玉杰
齐齐哈尔大学生命科学与农林学院，黑龙江齐齐哈尔161006 东北林业大学林学院，黑龙江哈尔滨150040
生物科学
偏肿革裥菌漆酶基因启动子克隆转录调控
Lenzites gibbosalaccase genepromoter cloningtranscriptional regulation
《华南农业大学学报》 2013 (4)
白腐菌锰过氧化物酶cDNA基因的克隆、分子结构分析与原核表达的研究
524-530,7
国家自然科学基金（30671700）；中国博士后科学基金（20090460866）；齐齐哈尔市科技局社会发展攻关项目（ SF-GG-201204）
评论