华南农业大学学报Issue(4)：564-568,5.
介导分泌表达猪圆环病毒2型Cap蛋白的重组杆状病毒的构建
Construction of a Recombinant Baculovirus Secreted Expression Vector in Cap Protein of Porcine Circovirus Type 2
摘要
Abstract
The objective of this study was to utilize baculovirus expression vector system to express capsid (Cap)protein (in secreted form)of porcine circovirus 2.The gene sequences encoding Cap protein fused with a C-terminal 6 Histidine tag were cloned into the baculovirus pFastBac TM Dual vector under the control of pH promoter .The authentic signal peptide of porcine circovirus type 2 ORF2 was substituted with the ecdysteroid UDP-glucosyltransferase (EGT) signal peptide.Plasmid was transformed into Escherichia coli DH10Bac competent cells to obtain the recombinant shuttle vector Bacmid .The Bacmid was transfected in-to Sf9 cells to produce the recombinant baculovirus .Indirect immunofluorescent assay and Western-blot in-dicated that the recombinant baculovirus could express Cap protein in infected Sf 9 cells.关键词
猪圆环病毒2型/ORF2基因/EGT信号肽/Cap蛋白/重组杆状病毒Key words
porcine circovirus 2/ORF2 gene/EGT signal peptide/Cap protein/recombinant baculovirus分类
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张春雷,靳立明,叶昱,张杰,朱俊,高又文,廖明,樊惠英..介导分泌表达猪圆环病毒2型Cap蛋白的重组杆状病毒的构建[J].华南农业大学学报,2013,(4):564-568,5.基金项目
863计划项目（2011AA10A209）；973计划项目（2011CB504701）；广州市“珠江科技新星专项”项目（2012J2200086）；公益性行业（农业）科研专项 （）
