华南农业大学学报Issue(1)：12-16,5.
体外诱导嗜水气单胞菌对喹诺酮类耐药及其耐药机制研究
Characterization of quinolone resistance mechanism of Aeromonas hydrophila selected in vitro
摘要
Abstract
[Objective] To investigate the effect of the sub-inhibitory concentration of quinolones on resist-ance to quinolones in Aeromonas hydrophila and its mechanisms .[Method] The quinolone sensitive strains of A.hydrophila from clinical and ATCC7966 were selected in vitro stepwise exposure to increasing concentration of nalidixic acid (NAL) and ciprofloxacin (CIP) on solid medium.The quinolone resist-ance-determining region ( QRDR) was sequenced to determine the mutation for quinolone resistance .The minimal inhibitory concentrations ( MICs) of selected and non-selected antimicrobials as well as MIC by efflux pump inhibition cyanide chlorophenyl hydrazone ( CCCP) of each strain were determined by broth microdilution method .The relationship between sensitivity varieties , mutations and efflux pump activities were analyzed .[Result and conclusion] The MICs of NAL and CIP increased 1 024-and 64 000-fold af-ter selection , respectively , while the MICs of other antimicrobials increased diversely .When MIC of NAL or CIP in A.hydrophila reached 16μg/mL or 32μg/mL, Asp87→Tyr or Ser83→Arg, mutations of Gy-rA were identified , but no mutations of ParC were found .After CCCP was added , only MICs of fluoro-quinolones caused a slight decrease , implying that it might include the quinolone mechanisms of target gene mutations and an active efflux system in A.hydrophila.关键词
嗜水气单胞菌/喹诺酮类/体外诱导/基因突变/外排作用Key words
Aeromonas hydrophila/quinolone/selection in vitro/mutation/efflux分类
农业科技引用本文复制引用
邓玉婷,薛慧娟,姜兰,谭爱萍,吴雅丽,王伟利,罗理,赵飞..体外诱导嗜水气单胞菌对喹诺酮类耐药及其耐药机制研究[J].华南农业大学学报,2014,(1):12-16,5.基金项目
公益性行业（农业）科研专项（201203085）；广东省鱼病防治专项资金（2012年）；广东省科技计划项目 （）
