华南农业大学学报Issue(1)：43-48,6.
转 phyA 基因玉米新种质的创制
Development of corn new germplasm with transgenic phyA
摘要
Abstract
[Objective]To construct the vector of phytase gene ( phyA) specific expression in roots and to develop transgenic maize with high phosphorus efficiency .[Method] Using PCR method , the expression cassette ZmGLU1P-phyA-Nos was constructed by amplifying phyA from its expression vector pCAM-BIA3301 .The expression cassette was inserted into the transitional vector pCAMBIA 1301-BADH with salt-tolerance BADH gene, which formed pCAMBIA1301-ZmGLU1P-phyA-Nos, the expression vector of phyA specific expression in roots .The phyA was transformed into maize embryogenic callus via Agrobacte-rium tumefaciens-mediated method .The regeneration plants were detected by molecular biology tech-niques .[Result and conclusion] The transformed plants were detected by PCR and 9 transgenic lines were obtained.Through salt-resistant selection, PCR, Southern blotting and RT-PCR analysis of T1 gen-eration plants , the results showed that phyA were successfully integrated into the maize genome and 6 transgenic lines were obtained .The analysis of phytase activity in maize plant root of T 2 generation plants showed that the phytase was able to be expressed highly and secreted from roots .The phytase activity of transformed plants increased 10.9 times on average, with the highest phytase activity being 5.432U/g. The new corn germplasm with transgenic phytase was obtained .Moreover , this study could lay a founda-tion for creation of new corn germplasm with high phosphorus efficiency and high maize yield .关键词
玉米/植酸酶基因/表达载体构建/新种质Key words
maize/phyA/construction of expression vector/new germplasm分类
农业科技引用本文复制引用
刘强,张丽媛,马红丹,赵邯郸,袁文娅,刘思言,关淑艳..转 phyA 基因玉米新种质的创制[J].华南农业大学学报,2014,(1):43-48,6.基金项目
国家转基因专项（20112x11003-005）；吉林省科技发展计划项目（20120215）；吉林农业大学校内启动基金（201242）；国家农业科技成果转化资金项目 （）
