华南农业大学学报Issue(1)：73-78,6.
春大豆花芽 cDNA 文库的构建及质量分析
Construction and quality analysis of cDNA library from flower buds of spring soybean cultivars
摘要
Abstract
[Objective] To understand soybean pods and multigrain gene regulation mechanisms of impro-ving soybean production , a soybean flower bud cDNA library was constructed .The quality of library con-struction was initially identified according to the requirements .[Method]In order to study the novel genes from flower bud mutants of soybean , a full-length cDNA library from flower bud mutants of soybean Jinong 18 was constructed .Total RNA from young flower bud mutants of soybean was extracted .Double strand cDNA was synthesized by SMART method .After proteinase K digestion and SfiⅠ digestion , the ds cDNA fragments were ligated to the λTriplEx vector .A cDNA library of soybean was successfully con-structed .[Result and conclusion]With the unamplified library stored after amplification , the titer of the amplified library was estimated as 2.13 ×108 pfu/mL and the recombination rate was approximately 95.3%.PCR results showed that the inserts varied from 0.5 to 2.0 kb with an average size of 1.0 kb or so.It indicated that this library could be used for full-length genes screening and cloning of low abundance genes.The library will lay a foundation for the genes clon and molecular biological research .关键词
大豆/花芽/cDNA文库构建/质量分析Key words
soybean/flower bud/cDNA library construction/quality analysis分类
生物科学引用本文复制引用
王楠,周莹,姚丹,尹俊琦,曲静,王丕武..春大豆花芽 cDNA 文库的构建及质量分析[J].华南农业大学学报,2014,(1):73-78,6.基金项目
吉林省科技支撑重点项目（20090203）；吉林省发改委科学技术研究项目 （）
