华南农业大学学报Issue(1):93-99,7.
罗汉果甜苷Ⅴ合成生理规律的研究
Physiological regularities of Siraitia grosvenorii mogrosideⅤbiosynthesis
摘要
Abstract
[Objective] To discuss physiological regularities of Siraitia grosvenorii mogrosideⅤbiosynthe-sis .[Method] Comparative experiments of variety , shading , gas storing and low-temperature storing were carried out .Through these experiments , correlations between Siraitia grosvenorii mogrosides and saccha-rides metabolizing in fruits , effects of photosynthesis , respiration and temperature on them were analysed .[Result and conclusion] In 30 d fruits, mogrosides were mainly mogroside ⅡE andⅢ, sucrose and glu-cose content were low .During 30-70 d, mogroside ⅡE and Ⅲcontent rapidly decreased;sucrose and glucose content increased; mogroside Ⅴ occrred, and its content sharply rose to near the peak value . During 70-90 d, mogroside Ⅴ was what only detected and found among mogrosides; sucrose content continually increased;glucose content obviously decreased .The higher photosynthetic rates of the varie-ty, the higher its mogroside Ⅴand glucose content .In shading conditions , photosynthetic rate of leaves decreased , but mogroside Ⅴand glucose content of fruits both climbed up .Both gas storing and low-tem-perature storing improved glucose accumulation but inhibited mogroside Ⅴbiosynthesis .These studies sug-gested that low saccharide group mogrosides gradually combined glucoses into mogroside Ⅴ.It did not im-prove mogrosideⅤbiosynthesis that leaf photosynthesis and fruit respiration were regulated to increase glu -cose amount at a late developmental stage .These results showed that low saccharide group mogrosides a-mount and key enzymatic activities , not the glucose amount ,were possibly the main factors of limiting mo-grosideⅤbiosynthesis .关键词
罗汉果/甜苷Ⅴ/葡萄糖/光合速率/酶活性Key words
Siraitia grosvenorii/mogroside Ⅴ/glucose/photosynthetic rate/enzymatic activity分类
农业科技引用本文复制引用
莫长明,王海英,马小军,唐其,万凌云,翟勇进..罗汉果甜苷Ⅴ合成生理规律的研究[J].华南农业大学学报,2014,(1):93-99,7.基金项目
国家自然科学基金(81373914);国家科技支撑计划项目(2011BA101B03);广西科技攻关项目 ()