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罗汉果甜苷Ⅴ合成生理规律的研究

莫长明 王海英 马小军 唐其 万凌云 翟勇进

华南农业大学学报Issue(1):93-99,7.
华南农业大学学报Issue(1):93-99,7.

罗汉果甜苷Ⅴ合成生理规律的研究

Physiological regularities of Siraitia grosvenorii mogrosideⅤbiosynthesis

莫长明 1王海英 2马小军 3唐其 4万凌云 1翟勇进5

作者信息

  • 1. 广西大学农学院,广西南宁530004
  • 2. 广西药用植物园广西药用资源保护与遗传改良重点实验室,广西南宁530023
  • 3. 中国医学科学院药用植物研究所,北京100193
  • 4. 天津中医药大学中药学院,天津300193
  • 5. 中国医学科学院药用植物研究所,北京100193
  • 折叠

摘要

Abstract

[Objective] To discuss physiological regularities of Siraitia grosvenorii mogrosideⅤbiosynthe-sis .[Method] Comparative experiments of variety , shading , gas storing and low-temperature storing were carried out .Through these experiments , correlations between Siraitia grosvenorii mogrosides and saccha-rides metabolizing in fruits , effects of photosynthesis , respiration and temperature on them were analysed .[Result and conclusion] In 30 d fruits, mogrosides were mainly mogroside ⅡE andⅢ, sucrose and glu-cose content were low .During 30-70 d, mogroside ⅡE and Ⅲcontent rapidly decreased;sucrose and glucose content increased; mogroside Ⅴ occrred, and its content sharply rose to near the peak value . During 70-90 d, mogroside Ⅴ was what only detected and found among mogrosides; sucrose content continually increased;glucose content obviously decreased .The higher photosynthetic rates of the varie-ty, the higher its mogroside Ⅴand glucose content .In shading conditions , photosynthetic rate of leaves decreased , but mogroside Ⅴand glucose content of fruits both climbed up .Both gas storing and low-tem-perature storing improved glucose accumulation but inhibited mogroside Ⅴbiosynthesis .These studies sug-gested that low saccharide group mogrosides gradually combined glucoses into mogroside Ⅴ.It did not im-prove mogrosideⅤbiosynthesis that leaf photosynthesis and fruit respiration were regulated to increase glu -cose amount at a late developmental stage .These results showed that low saccharide group mogrosides a-mount and key enzymatic activities , not the glucose amount ,were possibly the main factors of limiting mo-grosideⅤbiosynthesis .

关键词

罗汉果/甜苷Ⅴ/葡萄糖/光合速率/酶活性

Key words

Siraitia grosvenorii/mogroside Ⅴ/glucose/photosynthetic rate/enzymatic activity

分类

农业科技

引用本文复制引用

莫长明,王海英,马小军,唐其,万凌云,翟勇进..罗汉果甜苷Ⅴ合成生理规律的研究[J].华南农业大学学报,2014,(1):93-99,7.

基金项目

国家自然科学基金(81373914);国家科技支撑计划项目(2011BA101B03);广西科技攻关项目 ()

华南农业大学学报

OA北大核心CSCDCSTPCD

1001-411X

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