华南农业大学学报Issue(2)：66-72,7.
生物絮凝高活性菌株筛选及发酵优化
Screening bioflocculant strains and optimization of fermentation
摘要
Abstract
[Objective] This study aimed at isolating and screening of efficiently biological flocculation strains from the paddy field of the planted wild rice .[Method]A high bioflocculant strain YH 39 was ob-tained from the screening of kaolin suspension flocculation effects of 135 strains isolated from Oryza offici-nalis on VM medium.[Result and conclusion]16S rDNA sequences analysis showed that the strain YH39 was closely related to the known species Burkholderia cepacia with 98.6%similarity.The best car-bon and nitrogen sources for producing flocculation were glucose and potassium nitrate , respectively .The fermentation kinetics analysis showed that strain YH 39 reached the highest values of biomass concentra-tion and flocculating activity in 16 h.Flocculant composition analysis indicated that the bioflocculant components were polysaccharide with good heat resistance , which was heated at 60℃for 30 min without losing flocculating activity .The application of bioflocculation of three kinds of dyeing wastewater show that the fermentation liquid has good flocculating effects on dyeing wastewater with effective decolorization and reduces COD Cr in dyeing wastewater .关键词
微生物絮凝剂/絮凝活性/菌株筛选/发酵优化Key words
microbial flocculant/flocculating activity/screening strain/optimization of fermentation分类
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彭桂香,卢秋雁,孔慧清,李雄,万涛,卢钰升,谭志远..生物絮凝高活性菌株筛选及发酵优化[J].华南农业大学学报,2014,(2):66-72,7.基金项目
973计划项目（2010CB126502）；国家自然科学基金（31370052）；广东省科技计划项目（2010B090400434，2012B010300018）；农业部南方植物营养与肥料重点实验室和广东省养分资源循环利用与耕地保育重点实验室开放基金资助项目（TFS2011-04）；广东省烟草专卖局资助项目 （）
