华南农业大学学报Issue(3)：13-17,5.
乌梅等20种中药对胸膜肺炎放线杆菌的体外抗菌活性研究
A study on antibacterial activity of crude extract from twenty traditional Chinese medicines like Fructus mume against Actinobacillus pleuropneumoniae in vitro
摘要
Abstract
[Objective]The antibacterial activity of twenty traditional Chinese medicines against Actinoba-cillus pleuropneumoniae in vitro was evaluated in this study .[Method]Twenty traditional Chinese medi-cines like Fructus mume were extracted by ethanol reflux extraction ,water decoction and ultrasonic wave . Minimal inhibitory concentrations ( MICs) of twenty traditional Chinese medicines against A.pleuropneu-moniae were determined by the macrodilution broth method .The joint antimicrobial activity of some tradi-tional Chinese medicines was evaluated in vitro.[Result and conclusion]The results showed that the MICs of the extract obtained from F.mume, Rhizoma coptidis, Terminalia chebula, Cortex fraxini, Sanguisorbae officinalis and Polygonum cuspidatum against A.pleuropneumoniae ranged from 6.25 to 50.00 mg/mL. MICs of the extract obtained from Isatis indigotica, Artemisia capillaris,Glycyrrhiza uralensis and Dictam-nus dasycarpus ranged from 50.00 to 100.00 mg/mL.MICs of the extract obtained from Phellodendron amurense, Eucommia ulmoides, Lonicera japonica, Bupleurum chinense, Taraxacum mongolicum, Baphi-cacanthus cusia, Gardenia jasminoides and Astragalus membranaceus were higher than 100.00 mg/mL. The results of joint bacteriostatic test showed that the fractional inhibitory concentration index ( FICI) of the pairwise combination of F.mume, R.coptidis, Te.chebula and Po.cuspidatum was lower than or equal to 1, and FICI of C.fraxini with Po.cuspidatum and F.mume was higher than 2.The F.mume, R.coptidis, Te.chebula, Po.cuspidatum, C.fraxini and S.officinalis had a good antimicrobial activi-ty.The pairwise combination of F.mume, R.coptidis, Te.chebula and Po.cuspidatum showed an en-hanced or additive synergy action , and C.fraxini showed antagonistic action with F.mume and Po.cus-pidatum, respectively .关键词
乌梅/黄连/诃子/虎杖/胸膜肺炎放线杆菌/抗菌活性Key words
Fructus mume/Rhizoma coptidis/Terminalia chebula/Polygonum cuspidatum/Actinobacillus pleuropneumoniae/antimicrobial activity分类
农业科技引用本文复制引用
康帅,殷中琼,贾仁勇,代如意,李莉,彭练慈,曲径,梁陈..乌梅等20种中药对胸膜肺炎放线杆菌的体外抗菌活性研究[J].华南农业大学学报,2014,(3):13-17,5.基金项目
“十二五”农村领域国家科技计划项目（2011BAD34B03）四川省青年科技创新研究团队 （）
