华南农业大学学报Issue(3)：18-23,6.
鸽TLR7基因的克隆、鉴定及表达分析
Molecular cloning, characterization and expression analysis of the pigeon toll-like receptor 7 gene
摘要
Abstract
[Objective]To clone and characterize the pigeon Toll like receptor 7 (TLR7) gene.[Method]RT-PCR,RACE ( rapid amplification of cDNA ends ) ,Quantitative real-time PCR and bio-informatics soft-ware was used in this study .[Result and conclusion] The study showed that the full-length of pigeon TLR7 cDNA sequence was 3 516 bp with the longest open reading frame ( ORF) of 3 175 bp encoding a peptide of 1 048 amino acids .The deduced amino acid sequence of pigeon TLR7 contained three main structural domains including an extracellular leucine rich repeats domains (LRRs),a transmembrane domain (TM) and a Toll/IL-1 receptor domain ( TIR);pigeon TLR7 shared amino acid sequence similarity with the Anser cygnoides TLR7 gene, Anas platyrhynchos TLR7 gene, Gallus gallus TLR7 gene (above 78%), mammal TLR7 gene ( about 60%) , and fish TLR7 gene ( less than 55%) .Quantitative real-time PCR analysis re-vealed that the pigeon TLR7 mRNA widespread expression in the tested tissues was high in small intestine , spleen, kidney and low in brain,lung, trachea, heart, pancreas, muscle and skin.In this study TLR7 gene has been isolated from pigeon , cloned and its main functional domains have been predicted .关键词
鸽/TLR7基因/序列分析/受体表达Key words
pigeon/TLR7 gene/sequence analysis/receptor expression分类
农业科技引用本文复制引用
韩翡,任涛,焦培荣,韦良孟,何静,宋亚芬,康银峰,崔进,张烁,廖明..鸽TLR7基因的克隆、鉴定及表达分析[J].华南农业大学学报,2014,(3):18-23,6.基金项目
国家自然科学基金（31172343，31072139，31372412）；公益性行业（农业）科研专项 （）
