河南农业科学Issue(12)：79-85,7.
细辛精油对2种农业害虫保护酶和解毒酶活性的影响
Effects of Asarum Essential Oils on Protection/Detoxification Enzyme Activities of Two Agricultural Pest Insects
摘要
Abstract
To evaluate the insecticidal potential and mechanism of asarum essential oils ,the effects of three kinds of asarum essential oils extracted by different methods on protection/detoxification enzyme activities of Asian corn borer[Ostrinia furnacalis(Guenee)] and army worm[Mythimna seperata(Walker)] were studied .The three asarum essential oils were as follows :essential oil X1 was extracted by steam distillation method ,essential oil X3 was extracted by supercritical CO2 ex-traction method ,and essential oil X5 was a product obtained by refining X3 through a silica gel column chromatography .X1 was sprayed on the corn sprout ,according to a dose of 1 000 mg/L . X3 and X5 were separately added to the corn borer artificial feed ,according to a dose of 0 .5 mg/g . The experiment tested the response of Asian corn borer larvae fed on artificial diet containing X 3 or X5 ,and response of army worm larvae fed on tender corn shoots containing X 1 .In control group ,asarum essential oil was not added .The results showed that ,under the set experimental concentrations ,essential oils X1 and X3 inhibited the activities of three protective enzymes (su-peroxide dismutase ,SOD ;catalase ,CAT ;peroxidase ,POD) of the test insects ;X5 showed cer-tain inhibitory effect on POD activity of the test insects ,but exhibited an activation effect on SOD and CAT .Asarum essential oils showed some activation effect on carboxylesterase (CarE) ,acid phosphatase (ApE) and glutathione S-transferase (GST ) ,but the degree of activation effect re-duced over time ,meanwhile alkaline phosphatase(ALP) activity significantly increased early ,then significantly reduced ,w hich demonstrated that insects firstly increased their detoxification ability under excessive exogenous compound ,and then the detoxification capacity would decrease with the accumulation of toxic substances .The insecticidal effect of asarum essential oils may be achieved by inhibiting the insects’ protective enzymes .关键词
细辛精油/农业害虫/超氧化物歧化酶/过氧化氢酶/过氧化物酶/保护酶/解毒酶Key words
asarum essential oils/agricultural pest insects/superoxide dismutase (SOD )/cata-lase (CAT)/peroxidase (POD)/protection enzyme/detoxification enzyme分类
农业科技引用本文复制引用
姬兰柱,王桂清,刘艳,王远遐,张悦,易雪梅..细辛精油对2种农业害虫保护酶和解毒酶活性的影响[J].河南农业科学,2013,(12):79-85,7.基金项目
沈阳市科技计划项目（F11264122）；辽宁省科技计划项目（2012214001）；山东省自然科学基金联合专项 （）
