河南农业科学Issue(1)：69-73,83,6.
棉花黄萎病菌 T-DNA插入突变体库的构建及致病缺陷突变体筛选
Construction of T-DNA Insertional Mutant Library for V erticillium dahliae and Identification of Pathogenicity Defective M utants
摘要
Abstract
The fungus Verticillium dahliae causes cotton Verticillium wilt ,one of the most devas-tating diseases of cotton ,however ,the molecular basis of pathogenicity of V .dahliae is unclear .In this study ,Agrobacterium tumefaciens-mediated transformation(ATMT)was applied for inser-tional mutagenesis of V .dahliae FGH2 ,a highly virulent and defoliating strain .The collection of 1 200 T-DNA random insertion transformants of V .dahliae was generated ,using the optimized cocultivating conditions of V .dahliae conidia and A grobacterium(1∶250 ratio )for 48 h ,with the AS concentration of 400 μmol/L and the nitrocellulose filter as the filter type in the co-cultiva-tion ,resulting in 612 .5 transformants per 106 conidia .T hree pathogenicity defective V .dahliae mutants were obtained through testing the pathogenicity of 300 T-DNA transformants on the cot-ton variety Yinshan 1 seedling by dipping roots with spore suspension ,including one T-DNA in-sertion mutant losing the ability for forming microsclerotia and one T-DNA insertion mutant de-fective of microsclerotia development capability .The T-DNA insertional mutagenesis library of V . dahliae lays a foundation for researching molecular mechanisms of pathogenicity and microsclero-tia development of V .dahliae .关键词
棉花黄萎病/大丽轮枝菌/农杆菌介导的遗传转化/T-DNA插入突变/致病性Key words
cotton Verticillium wilt/Verticillium dahliae/Agrobacterium tumefaciens-mediated transformation/T-DNA insertional mutagenesis/pathogenicity分类
农业科技引用本文复制引用
谷素静,汪敏,桑茜,赵静,李洪连..棉花黄萎病菌 T-DNA插入突变体库的构建及致病缺陷突变体筛选[J].河南农业科学,2014,(1):69-73,83,6.基金项目
国家自然科学基金项目（30700520）；河南省自然科学基金项目（2008A208015）；河南省教育厅科学技术研究重点项目 （）
